抑制survivin表达对前列腺癌细胞pc-3凋亡的影响  

Effect of inhibition of survivin expression on the apoptosis of prostate cancer cell pc-3

作　　者：黄谷[1] 张明[1] 王海坤[1] 

机构地区：[1]武警广东总队医院外三科,广东广州510507

出　　处：《武警医学院学报》2006年第1期14-16,共3页Acta Academiae Medicinae CPAPF

摘　　要：[目的]研究survivin与前列腺癌的关系,探讨survivin对前列腺癌细胞pc-3凋亡的影响及其机制。 [方法]运用RNA干扰技术阻断survivin在前列腺癌细胞pc-3中的表达,采用流式细胞计数等方法检测survivin 被阻断后,对前列腺癌细胞pc-3凋亡的影响。[结果]阻断survivin在前列腺癌细胞pc-3中的表达后,能够引起 pc-3细胞的凋亡,其机制可能是由于活化了凋亡通路的caspase-3。[结论]Survivin可以做为治疗前列腺癌的一个潜在的基因靶点。[Objective] To evaluate the relationship between survivin and prostate cancer and the effect of survivin on the apoptosis of prostate cancer and it＇s probable mechanism. [Methods] Survivin expression on prostate cancer cell pc-3 was interrupted by RNA interference （RNAi） technigae and the apoptosis ratio of prostate cancer cell PC-3 was determined by flow cytometer. [Results] Interruption of the survivin expression of prostate cancer cell pc-3 could induce the apoptosis of pe-3 cells. The mechanism might be that the apoptosis pathway of caspase-3 was activated. [Conclusion] Survivin might be act as a potential therapeutic target for prostate cancer.

关 键 词：RNA干扰 SURVIVIN PC-3细胞 凋亡 CASPASE-3 

分 类 号：Q255[生物学—细胞生物学] R979.1[医药卫生—药品]