HPLC测定利肝隆片中五味子甲素和五味子乙素含量  

Determination of deoxyschizandrin and schisandrin B in Liganlong Tablet by HPLC

作　　者：吕邵娃[1] 管庆霞[1] 李永吉[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中成药》2006年第5期662-664,共3页Chinese Traditional Patent Medicine

摘　　要：目的：建立高效液相色谱法同时测定利肝隆片（五味子，黄芪，当归，刺五加等）中五味子甲素和五味子乙素含量的方法。方法：采用高效液相色谱法。Waters PAD 2695—2996系统；Symmetry shield^TM RP18 5μm，3．9mm×150mm；流动相：甲醇-四氢呋喃-水（64：4：32）；流速1mL／min；检测波长：220nm；柱温30℃。结果：五味予甲素在0．068—0．340μg之间线性关系良好（r=0．9999）；五味子乙素在0．06—0．30μg之间线性关系良好（r=0．9998），理论塔板数n=3555．5，分离度R甲=5．09，R乙=1．12。结论：本法灵敏度高、操作简便、结果准确。可用于利肝隆片中五味子甲素和五味子乙素的含量测定。AIM： A quantitative method was developed for the determination of deoxyschizandrin and schisandrin B in Liganlong Tablet （Fructus Schisandrae Chinensis, Radix Astragali, Radix Angelicae Sinensis, Radix et Rhizoma seucaulis Acanthopanacis Senticosi, etc. ） by HPLC. METHODS： The chromatographic conditions included column Symmetry shield^TM RP18 5 μm, 3.9 mm × 150 mm, mobile phase： methanol-tetrahydrofuran-water （64： 4： 32）, flow rate at 1 mL/min, wavelength at 220 nm. RESULTS： The number of theoretical plates is 3555.5. The Deoxyschizandrin liner is 0. 068 ～ 0. 340 μg （ r = 0. 999 9） and the Schisandfin liner range is 0. 06～0. 30 μg （r =0. 999 8）. The resolutions are 5.09, 1.12, respectively. CONCLUSION： The method is sensitive, quick and accurate for the determination of deoxyschizandrin and schisandrin B in Liganlong Tablet.

关 键 词：利肝隆片 五味子甲素 五味子乙素 HPLC 

分 类 号：R927.2[医药卫生—药学]