活性污泥处理高浓度基质发酵产氢条件的优化  

Sludge for hydrogen production in high-concentration medium

作　　者：吴薛明[1] 申宁[1] 何冰芳[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《南京工业大学学报（自然科学版）》2006年第3期28-32,75,共6页Journal of Nanjing Tech University(Natural Science Edition)

基　　金："973"项目资助(2003CB716004);国家博士点专项基金(20040291007)

摘　　要：以蔗糖为基质模拟有机废水,通过对活性污泥的梯度驯化,提高了活性污泥对高糖溶液的代谢能力与产氢能力,在此基础上对其产氢条件进行优化。优化后发酵条件为:厌氧发酵温度36℃,初始pH值5.0,蔗糖90 g/L,玉米浆8 mL/L,FeSO4.7H2O 20 mg/L,MgC l2.6H2O 10 mg/L,K2HPO41.0 g/L。优化后的活性污泥平均产氢速率达到565 mL/(L.h),比优化前提高了223%;比产氢率为3.04 mol/mol,比优化前提高了30%。提出了有机氮与生长因子同步添加的高效廉价培养基方案,为活性污泥厌氧发酵生物制氢技术的工业化奠定了基础。The main focus of this study was on the effective hydrogen bio-production using sucrose medium as the simulated organic wastewater. The improvement of sucrose metabolism rate and hydrogen evolution efficiency was achieved throught batch acclimation from low-concentration to high-concentration of sucrose. The conditions of anaerobic fermentation of batch acclimated sludge were investigated. The optimized medium for anaerobic fermentation contains 90 g/L of sucrose, 8 mL/L of corn liquid steep, 20 mg/L of FeSO4 7H2O, 10 mg/L of MgCl2 6HzO and 1.0 g/L of K2HPO4. The medium was adjusted to pH of 5.0. Optimized fermentation temperature was 36 ℃. The average hydrogen evolution rate at optimized the initial conditions; The yield of hydrogen conditions was 565 mL H2/L·h, which was 223% higher than that at was 3.04 mol H2/mol sucrose, which was 30% higher than that at the initial conditions. The efficient medium which supplied organic nitrogen and suggested. This growth medium is of considerable economic significance for practical process sludge anaerobic fermentation.

关 键 词：生物产氢 活性污泥 厌氧发酵 

分 类 号：Q81[生物学—生物工程]