大鼠肝再生过程中肝细胞caspase-3和caspase-8活性的变化  

Changes of hepatic caspase-3 and caspase-8 activities during liver regeneration in rats

作　　者：周运恒[1] 缪明永[1] 李洋[1] 时多[1] 黄鹤[1] 王学敏[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础医学部生物化学与分子生物学教研室,上海200433

出　　处：《第二军医大学学报》2006年第5期500-502,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30070346)~~

摘　　要：目的:研究大鼠肝再生过程中肝细胞caspase-3和caspase-8活性的变化规律。方法:雄性SD大鼠70%肝切除(PH),制作肝再生模型,制备肝匀浆液,经离心后用酶标仪分别测定肝再生过程中不同时期(共8个时间点),肝细胞的凋亡酶活性。结果:Caspase-3活性,与对照组相比,在PH后3、6、72、120和168 h时显著性增高(P<0.001);而整个过程中Caspase-8的活性除了在PH后168 h时高于对照组外(P<0.05),其余时间点没有明显增高。结论:肝再生过程中肝细胞凋亡酶Caspase-3活性的升高可能主要是由内源性线粒体途径引起的。Objective：To study the changes of hepatic caspase-3 and caspase-8 activities during liver regeneration in rats. Methods： Liver regeneration model was produced by 70% partial hepatectomy （PH） in male SD rats. Then the rats were sacrificed and the liver homogenate were prepared. Hepatic caspase-3 and caspase-8 activities were determined at 8 different time points during the liver regeneration at the absorbance of 405 nm. Results： Hepatic caspase-3 activity increased significantly 3 h, 6,72 h, 120 h and 168 h after PH compared with that of control group（P〈0. 001）; Hepatic caspase-8 activity increased 168 h after PH（P%0.05） and had no obvious change at any other time points. Conclusion： Our result suggests that the increase of caspase-3 activity during the liver regeneration may be mainly induced by the mitochondrial pathway.

关 键 词：肝再生 肝细胞 CASPASE-3 CASPASE-8 

分 类 号：R657.3[医药卫生—外科学]