天冬中呋甾皂苷含量测定方法的建立  

A method for determination of furostanol saponins in saponins extract from Radix Asparagi

作　　者：沈阳[1] 陈海生[1] 徐从立[1] 徐红利[1] 

机构地区：[1]第二军医大学药学院天然药物化学教研室,上海200433

出　　处：《第二军医大学学报》2006年第5期548-549,共2页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(20472113).~~

摘　　要：目的：采用E试剂比色法建立了天冬总皂苷含量测定方法。方法：用对二甲氨基苯甲醛-HCl乙醇液的发色体系，以天冬皂苷AC-9作为指标，在波长510nm测定天冬总皂苷含量。结果：标准曲线在0．08～0．40mg范围内线性关系良好，平均回收率为99．79％（RSD=1．05％）。结论：此法可作为评价天冬总皂苷的质量标准。Objective：To establish an Ehrlich reaction-colorimetry method for determining furostanol saponins in saponins extract from Radix Asparagi. Methods： Based on the coloration reaction between furostanol saponins and p-dimethylaminobenzaldehyde, a colorimetry method was established to determine the content of furostanol saponins in saponins extract from Radix Asparagi at 510 nm. Saponins AC- 9 was measured as reference in the determination. Results： The standard curve of saponins AC-9 showed good linearity in the range of 0.08-0.40 mg and the average recovery was 99.79% （RSD= 1, 05% ）. Conclusion.. This method is rapid, accurate and easy to be carried out, and it can be used to control the quality of Radix Asparagi.

关 键 词：天冬 呋甾皂苷 E试剂 分光光度法 含量测定 

分 类 号：R282.71[医药卫生—中药学]