牛膝多糖对哮喘大鼠信号转导子和转录激活子6表达的影响  

EFFECT OF ACHYRANTHES BIDENTATA POLYSACCHARIDES ON SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 6 AND ITS mRNA EXPRESSION IN A RAT MODEL OF ASTHMA

作　　者：李昌崇[1] 叶乐平[1] 苏苗赏[1] 胡晓光[1] 张维溪[1] 罗运春[1] 

机构地区：[1]温州医学院附属育英儿童医院呼吸内科,浙江温州325027

出　　处：《中国应用生理学杂志》2006年第2期210-214,共5页Chinese Journal of Applied Physiology

基　　金：浙江省自然科学基金资助项目(301485)

摘　　要：目的:研究牛膝多糖(ABPS)对支气管哮喘大鼠支气管信号转导子和转录激活子6(STAT6)及其mRNA表达的影响。方法:雄性SD大鼠30只随机分为正常对照组、哮喘组和哮喘+牛膝多糖组(ABPS组)。留取支气管肺泡灌洗液(BALF)进行细胞总数、嗜酸性粒细胞(EOS)和分类计数;并测定BALF和血清中白细胞介素4(IL-4)浓度;采用免疫组化法和原位杂交法分别检测STAT6蛋白和STAT6 mRNA的表达。结果:①哮喘组BALF中细胞总数、EOS绝对值和EOS占细胞总数的百分比(EOS%)均高于对照组(P<0.01),ABPS组上述指标均低于哮喘组(P<0.01);②BALF和血清中IL-4浓度哮喘组均高于对照组(均为P<0.01),ABPS组均低于哮喘组(均为P<0.01);③免疫组化和原位杂交显示,哮喘组支气管STAT6蛋白和STAT6 mRNA表达的平均吸光度(LD)均高于对照组,ABPS组则均低于哮喘组(均为P<0.01),其主要表达细胞是上皮细胞。结论:哮喘大鼠支气管STAT6及其mRNA较强表达,上皮细胞是其主要表达细胞;牛膝多糖有抑制哮喘气道EOS性炎症的作用,下调STAT6及其mRNA表达,使IL-4合成减少可能为其重要作用机制。Aim： To study the effect of achyranthes bidentata polysaccharides（ABPS） on the expression of signal transducer and activator of transcription 6 and its mRNA in bronchus of a rat model of asthma. Methods： Thirty male SD rats were randomly divided into three groups： the control group, asthma group and ABPS group. The total cell numbers, eosinophils（EOS） numbers and differentiated cell numbers in bronchoalveolar lavage fluid（BALF） were counted by different count fluids. The concentrations of IL-4 in serum and BALF were measured by sandwich ELISA. The protein expressions of STAT6 were detected by immunohistochemistry techniques. The mRNA expressions of STAT6 were detected by hybridization in situ. Results： ①The total cell numbers in BALF, the absolute numbers of EOS, the ratios of eosinophils to the total cell numbers （EOS%） of asthma group were all significantly higher than those of the control group（ P 〈20.01 ）. The total cell numbers in BALF, the absolute numbers of EOS and EOS% of ABPS group were all significantly lower than those of asthma group （P 〈2 0.01）. ②The concentrations of IL-4 in BALF and serum of asthma group were significantly higher than those of control group （ P 〈2 0.01 ）, while the concentrations of IL-4 in BALF and serum of ABPS group were significantly lower than those of asthma group. ③Immunohistochemistry showed that the protein content of STAT6 around the bronchus of asthma group was significantly higher than that of the control group（ P 〈2 0.01 ）, while that of ABPS group was significantly lower than that of asthma group , the epithelial cells were the chief expression cells; hybridization in situ showed that the mRNA expression of STAT6 around the bronchus of asthma group was significantly higher than that of the control group （P 〈0.01 ）, while that of ABPS group was significantly lower than that of asthma group , the epithelial cells were the chief expression cells. Conclusion. STAT6 protein and STAT6 mRNA were found strongly expr

关 键 词：哮喘 信号转导子和转录激活子6 牛膝多糖 

分 类 号：R562.2[医药卫生—呼吸系统] R392.11[医药卫生—内科学]