马传染性贫血病毒非编码区LTR嵌合克隆的生物学特性  

Characterization of a LTR Chimeric Infectious Clone of Chinese Equine Infectious Anemia Virus

作　　者：魏丽丽[1] 王晓钧[1] 耿庆华[1] 童骁[2] 粱华[2] 沈韬[2] 张晓燕[2] 相文华[1] 邵一鸣 沈荣显[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所 [2]中国疾病预防控制中心性病艾滋病预防控制中心,北京100050

出　　处：《中国病毒学》2006年第3期244-248,共5页Virologica Sinica

基　　金：国家自然科学基金青年基金资助(30200200)

摘　　要：将已构建的马传染性贫血病毒LTR强弱毒嵌合克隆衍生毒vLGFD9-12体内接种健康试验马,在150d观察期内,各组试验动物体症均未见异常。血液学分析发现,vLGFD9-12嵌合克隆衍生病毒与亲本弱毒疫苗株的白细胞与血红蛋白含量总体上没有明显的规律性的变化。在动物外周血中均检测到一定的病毒RNA拷贝数,但拷贝数较低。二者在诱导EIAV特异性淋巴细胞增殖功能和特异性细胞毒性杀伤反应中,亦具有相似的变化趋势和效应。本项研究为进一步确定我国马传贫弱毒疫苗株毒力致弱及免疫保护的分子机制奠定了重要的分子生物学基础。Equine infectious anemia virus full-length-gene chimeric clone derived virus, vLGFD9-12, was inoculated into healthy horses. Over an observation period of 150 days, all inoculated groups showed no overt abnormal symptoms. The results from routine blood assays showed that the counts of WBC and HGB had no obvious changes between vLGFD9-12 group and its parental virus pLGFD3-8. Low levels of viral RNA in the plasma of vLGFD9-12- and pLGFD3-8-infected horses was detected, The results from cytolytic T lymphocyte （CTL） and lymphocyte proliferation assay （LPA） showed that all inoculated animals had similar changes on virus-specific CTL responses and virus-specific lymphocyte proliferation activity. These results provide an important foundation for further study on mechanism of attenuation of the EIAV and the protective immunity against EIAV.

关 键 词：马传染性贫血病毒 衍生病毒 LTR 

分 类 号：S852.65[农业科学—基础兽医学]