墨汁灌注和血管铸型方法检测山羊胫骨血管应用性能的不同  

Comparison of ink intravascular injection and vascular cast in vascular detection of caprine tibia

作　　者：唐光辉[1] 裴国献[1] 王兴海[2] 陈滨[1] 曾宪利[1] 程文俊[1] 任义军[1] 魏宽海[1] 

机构地区：[1]南方医科大学南方医院创伤骨科中心,广东省广州市510515 [2]南方医科大学人体解剖学实验室,广东省广州市510515

出　　处：《中国临床康复》2006年第21期94-96,i0005,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家高科技研究发展计划重点项目(2003AA205010);广东省自然科学基金(04300723);广州市科委攻关重点项目(200221-0038)~~

摘　　要：目的:比较血管墨汁灌注和甲基丙烯酸甲酯血管铸型两种方法在山羊胫骨血管检测中的优缺点,为组织工程骨血管化检测寻找一种直观可行的方法。方法:实验于2004-05/2005-07在南方医科大学南方医院创伤骨科骨组织工程实验室完成。①12只正常中国青山羊随机分为墨汁灌注组,甲基丙烯酸甲酯血管铸型灌注组,每组6只。②墨汁灌注组采用中华墨汁、甲醛及生理盐水体积比为3∶1∶6的复合墨汁,行双侧股动脉插管,经血管冲洗后进行灌注,低温放置24h后取下胫骨标本行进一步处理,甲基丙烯酸甲酯血管铸型灌注组,每只山羊均行双侧后肢股动脉插管,血管彻底冲洗后将预聚后的甲基丙烯酸甲酯灌注,低温放置24～28h后取下胫骨标本行下一步处理。③灌注后两组标本分别采用大体解剖,未脱钙骨磨片和脱钙后组织切片等方法观察青山羊胫骨血管的走行与分布。结果:①山羊胫骨经墨汁灌注后大体解剖、未脱钙骨磨片和脱钙骨组织切片观察可清楚显示骨膜、骨皮质和骨髓腔血管;可以观察骨组织的显微结构,但不便于直观地观察胫骨与周围血管的空间立体结构。②血管铸型后的山羊胫骨制作的大体解剖标本,在胫骨中段制作2cm×1cm骨窗,可见髓腔内的铸型血管,骨皮质横、纵切面上可见微细的铸型血管断面;可以直观的观察山羊胫骨与周围血管的空间立体构筑,显示胫骨营养血管的分布、走行,未脱钙磨片能够清楚显示骨皮质血管网,扫描电镜可以观察到哈佛氏管内血管。结论:血管墨汁灌注适合用于微血管检测,便于染色后同时观察骨组织的显微结构;而血管铸型标本则适合大体观察血管的空间立体构筑,较为直观,两者在组织工程骨血管化检测中有不同的应用价值。AIM： To compare the advantage and disadvantage of ink intravascular injection and methyl methacrylate （MMA） vascular cast in the vascular detections of normal caprine tibia, and probe into the direct and feasible way of vessel detection in tissue-engineered bone. METHODS： The experiment was conducted in the Bone Tissue Engineering Laboratory of Traumatic Orthopedics Department, Nanfang Hospital of Southern Medical University between May 2004 and July 2005. ①Twelve normal Chinese caprines are randomly divided into 2 groups （6 in each）： ink intravascular injection group and MMA vascular cast group. ② In the ink group, the complex ink were consisted of Chinese ink, formaldehyde and saline in the proportions of 3：1：6. Femoral artery intubations were conducted in rats which was performed the perfusion after rinsed via tissues. After placed for 24 hours at the low temperature, the tibia samples were taken down to perform next treatment. In MMA group, each caprine was given femoral artery incubation in hindlimb and filled with MMA after the vessels were washed completely. The tibia samples were taken down and placed at low temperature for 24-28 hours to perform next treatment. ③After perfusion, gross anatomizatian, bone grinding sections without decalcification and decalcificated tissue sections were used respectively to observe the vascular distribution of the caprine tibia in both groups. RESULTS：. ① Gross anatomy, bone grinding sections without decalcification and decalcificated sections showed the vessels of periost, cortical bone and medullary cavity very clearly after the caprine tibias were perfused with Chinese ink. The microstructures of osseous tissues were observed, but it was not convenient to directly observe the threedimensional structures of tibias and surrounding tissues. ②The bone windows with the size of 2 cm×1cm were obtained from the vascular cast gross anatomy sample, and revealed the vascular cast in the medullary cavity and tiny crosses in transverse and longitud

关 键 词：组织工程 血管/解剖学和组织学 血管/超微结构 山羊 胫骨 

分 类 号：R318[医药卫生—生物医学工程]