GPⅠa／Ⅱa和GPⅣ在血小板-胶原黏附中的功能  

The functions of GPⅠa/Ⅱa and GPⅣin platelet adhesion to collagen

作　　者：张卓[1,2] 丛玉隆[1] (?)植光 

机构地区：[1]中国人民解放军总医院临床检验科,北京100853 [2]重庆医科大学检验系

出　　处：《临床检验杂志》2006年第3期193-195,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的探讨GPⅠa／Ⅱa和GPⅣ在血小板-胶原黏附过程中的功能。方法采用流式细胞术,在有或无抗GPⅠa／Ⅱa抗体、GPⅣ抗体阻断条件下,用抗CD42b-PE标识血小板并检测血小板与FTTC标记胶原黏附过程中FITc荧光强度的变化。结果抗GPⅠa／Ⅱa抗体能抑制血小板与胶原的黏附,对活化态血小板的抑制作用更明显(P<0．01);抗GPⅣ抗体在黏附早期能延迟黏附进程(P<0．05),但最终不能抑制血小板与胶原的黏附过程(P>0．05)。结论GPⅠa／Ⅱa在血小板与胶原黏附过程中有重要作用,阻断GPⅠa／Ⅱa能降低血小板对胶原的结合;GPⅣ在血小板与胶原黏附过程的早期有辅助加速作用。Objective To study the functions of GP Ⅰa/Ⅱa and GPⅣ during the course of platelet adhesion to collagen. Methods Reaction system which contains anti-GP Ⅰa/Ⅱa antibody, anti-GPⅣ antibody or no blocking antibody was analyzed by flow cytomety. PE labeled anti-CD42b antibody was used to select platelet and fluorescence intension of fluorescein isothiocyanate （FITC） was detected during the course of platelet adhesion to FITC-labeled collagen. Results The antibody against GP Ⅰa/Ⅱa inhibited platelet adhesion to collagen, especially to activated platelet （P 〈 0.01 ）. The antibody against GPIV delayed the course of platelet adhesion to collagen duringearly stage（P 〈0.05）,but it did not inhibit platelet adhesion to collagen at last（P 〉0.05）. Conclusion GP Ⅰa/Ⅱa plays an important roles in platelet adhesion to collagen. Blocking GP Ⅰa/Ⅱa may decrease the adhesion. GP IV may be helpful for acceleration ofplatelet adhesion to collagen at early stage.

关 键 词：血小板 GP Ⅰa/Ⅱa GPⅣ 胶原 流式细胞术 

分 类 号：R554[医药卫生—血液循环系统疾病]