内蒙古地区汉族人群TNFβ基因多态性与IgA肾病的相关性  

Study on relationship between polymorphism of TNF-βand IgA nephropathy of Han nationality Chinese in inner Mongolia Autonomous Region

作　　者：王程 王彩丽[2] 高丽君 雎天林 秦文斌 

机构地区：[1]内蒙古基因诊断研究所 [2]包头医学院第一附属医院肾内科,内蒙古包头014010

出　　处：《临床检验杂志》2006年第3期203-205,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的研究内蒙古地区汉族人群IgA肾病与TNFβ基因多态性的关系。方法用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对79例IgA肾病患者及80例正常对照TNFβ1 069位点等位基因A→G单碱基突变多态性进行分析。结果内蒙古地区汉族IgA肾病患者TNFβ*1／1纯合子和TNFβ*2／2纯合子检出率与正常对照组比较均有较大差异(均P< 0．05);TNFβ*2等位基因频率(68．99％)较正常人(53．75％)显著升高(P<0．01)。结论TNFβ*2基因可能与IgA肾病易患性相关。Objective To study the relationship of tumor necrosis factor beta （TNF-β） polymorphism with human IgA nephropathy ofChinese Han nationality in Inner Mongolia Autonomous Region. Methods The A→G single base mutation polymorphism in TNF-β1096 locus were analyzed among 80 normal controls and 79 IgA nephropathy patients by using polymerase chain reaction-restriction fragmentlength polymorphism （PCR-RFLP）. Results The genotype frequencies of TNFβ ＊ 1/1 and TNFβ ＊ 2/2 in IgA nephropathy patientswere significantly higher than that in normal controls （X^1/1 = 5.58 ,P 〈 0.01;X^2 2/2 =4.76,P 〈0.01 ）. The gene frequency of the TNFβ 2 in IgA nephropathy patients （68.99%） was significantly higher than that in normal controls （53.75 %, X2 = 7.78, P 〈 0.01,R^1 =1.91 ）. Condusion TNFβ＊ 2 allelic frequency is associated with IgA nephropathy.

关 键 词：肿瘤坏死因子 Β基因多态性 IGA肾病 

分 类 号：Q78[生物学—分子生物学]