小麦体细胞无性系895004与供体亲本的抗赤性、农艺性比较和RAPD分析  

Comparison of Resistance to Scab,Agronomic Traits and RAPD Analysis between the Somaclonal Wheat Line 895004 and Its Donor Parent

作　　者：沈晓蓉[1] 陆维忠[1] 许仁林[1] 蒋宁[1] 周淼平[1] 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所,南京210014

出　　处：《江苏农业学报》1996年第1期7-10,共4页Jiangsu Journal of Agricultural Sciences

基　　金：国家"863"高技术发展计划资助项目

摘　　要：小麦高产抗赤霉病品系895004是宁麦3号通过幼穗培养得到的体细胞无性系。通过田间性状观察和室内考种,其农艺性状和赤霉病的抗性均比宁麦3号有明显的改善。用140个随机引物对895004及其亲本宁麦3号进行 RAPD 分析,发现6个引物在两个品种间有多态性,因此认定895004在 DNA 分子水平上发生了变异。The wheat line 895004 is a somaclonal line with high yield and resistance to scab selected from the cultivar Ningmai 3 via in vitro culture of young spikes.Its agronomic traits,yielde traits and resistance to scab are improved obviously compared to Ningmai 3.To verify the varia- tion,140 random primers were used to amplify DNA from both 895004 and Ningmai 3.Six primers showed polymorphisms between 895004 and Ningmai 3,which suggests that changes have taken place at the DNA level.

关 键 词：小麦 赤霉病 体细胞无性系 变异 RAPD 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]