高效液相色谱法测定氢溴酸加兰他敏血药浓度  

Determination of galanthamine hydrobromide by HPLC

作　　者：刘新宇[1] 吴芳[1] 赵斌[2] 李君良[2] 

机构地区：[1]广东医学院附属福田医院,深圳518033 [2]广东医学院附属医院,湛江524001

出　　处：《中南药学》2006年第3期201-204,共4页Central South Pharmacy

摘　　要：目的 建立氢溴酸加兰他敏的血药浓度监测方法。方法采用碱化后有机溶剂提取的方法处理样品，运用高效液相色谱法进行测定，采用Hypersil-ODS2（200mm×4．0mm，5μm）色谱柱，以乙腈-水-四氢呋喃（8：95：5）为流动相，紫外检测波长224nm。结果氢溴酸加兰他敏血浆样品在1．26～126．00ng·mL^-1线性关系良好（r=0．9996，n=5），最低定量浓度为1．26ng·mL^-1，相对回收率〉98．0％，RSD〈6．0％。结论本法简便，准确，重现性好，可用于氢溴酸加兰他敏临床治疗血药浓度监测。OBJECTIVE To establish an HPLC method for the determination of clinical therapeutic concentration of galanthamine hydrobromide dispersion tablets in human plasma. METHODS The alkalified plasma samples were extracted by liquid-liquid extraction and analyzed on a Hypersil-ODS2 （200 mm×4.0 mm, 5 μm） with acetonitrile-water-tetrahydrofuran （8 ： 95 ： 5） as the mobile phase and its pH was adjusted to 3.0 with phosphoracid. The detection wavelength was 224 nm RESULTS The analytical results demonstrated a good linearity within 1.26-126.00 ng · mL^-1 （r=0. 999 6） for galanthamine. The recoveries were more than 98.0% and the inter-day and intra-day RSD were less than 6.0%. CONCLUSIONS This method is simple, accurate and reproducible and has been applied successfully in the quantitative determination of galanthamine.

关 键 词：氢溴酸加兰他敏 高效液相色谱法 治疗药物监测 

分 类 号：R917[医药卫生—药物分析学]