高效液相色谱法同时测定田基黄注射液中槲皮苷、异槲皮苷的含量  

Simultaneous Assaying of Quercitrin and Isoquercetin in Hypericum Japonicum Thunb.Injection by HPLC

作　　者：林焕泽[1] 吴秀荣[1] 

机构地区：[1]广东省茂名市人民医院药剂科,茂名市525000

出　　处：《中国药房》2006年第10期777-778,共2页China Pharmacy

摘　　要：目的:建立以高效液相色谱法同时测定田基黄注射液中槲皮苷、异槲皮苷含量的方法。方法:色谱柱为MerckLichrospher RP18,流动相为乙腈-(pH=3.0)磷酸盐缓冲液(18∶82),流速为1ml/min,柱温为25℃,检测波长为350nm。结果:槲皮苷、异槲皮苷进样量分别在0.278μg～4.440μg(r=0.9991)、0.315μg～2.520μg(r=0.9991)范围内与峰面积积分值线性关系良好;平均加样回收率分别为98.60%(RSD=2.23%)、97.79%(RSD=1.74%)。结论:本方法准确可靠、应用性强,可用于本品的质量控制。OBJECTIVE：To establish a HPLC method for the simultaneous determination of quercitrin and isoquercetin in hypericum japonicum thunb .injection.METHODS： Merck Lichrospher RP18 was used as chromatographic column and the mobile phase consisted of acetonitrile （pH = 3.0） buffer phosphate（ 18 ： 82） with flow rate at 1ml/min, column temperature at 25℃ and detection wavelength at 350nm.RESULTS： The linear range for quercitrin and isoquercetin were 0.278μg-4.440μg （r = 0.9 991） and 0.315μg-2.520μg（r= 0.9 991）, respectively.The average recovery were 98.60% （RSD= 2.23% ） and 97.79% （RSD = 1.74%）, respectively.CONCLUSION： This method is accurate, reliable and applicable, and suitable for the quality control of hypericum jponicum thunb.iniection.

关 键 词：高效液相色谱法 田基黄注射液 槲皮苷 异槲皮苷 含量 

分 类 号：R927.2[医药卫生—药学]