自体骨髓单个核细胞心肌移植治疗猪慢性缺血性心脏病的实验研究  

Improvement of Cardiac Function and Promotion of Angiogenesis after Transplantation of Autologous Bone Marrow Mononuclear Cells to Chronic Ischemic Myocardium in Swine

作　　者：祝曙光[1] 熊利华[1] 谢鸿发[2] 萧颂华[2] 华平[1] 廖洪映[1] 

机构地区：[1]中山大学附属第二医院胸心外科,广东广州510120 [2]香港大学心血管疾病研究所

出　　处：《中山大学学报（医学科学版）》2006年第3期293-296,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：卫生部科研基金资助项目(981093)

摘　　要：目的探讨自体骨髓单个核细胞心肌移植对猪慢性缺血心肌心功能的改善和促进血管新生的作用。方法小型猪左侧开胸在左冠状动脉回旋支起始部放置Ameroid环,4周后进行心脏超声波检测、冠状动脉造影和自体骨髓单个核细胞心肌移植,移植后4周进行心脏超声波检测,免疫组化计数血管密度。结果17只猪中有16只猪术后存活8周以上,活体冠状动脉造影显示左冠状动脉回旋支均闭塞。自体骨髓单个核细胞心肌移植组比对照组心功能有显著改善(P﹤0.05),移植组的血管数密度显著高于对照组(P﹤0.05),差异有统计学意义。结论自体骨髓单个核细胞心肌移植可改善慢性缺血心肌的心功能,其机理之一是通过改善缺血心肌的血供来实现。[Objective] To investigate the angiogenie effect and cardiac function improvement after intramyocardial transplantation of the autologous bone marrow mononuclear ceils to chronic isehemic myocardium in swine. [Method] Experimental swines underwent thoracotomy and placement of an Ameroid constrictor on the left circumflex branch of coronary artery. Four weeks later, cardiac function was examined by ecbocardiography, coronary arteriography was completed and autologous bone marrow mononuclear ceils was administrated directly into the myocardium in the circumflex branch distribution area. Another four weeks later, cardiac function was examined by echocardiography. The number of microvessel was also counted. [Result] Among 17 operated swines, 16 swines survived more than 8 weeks. Complete occlusion of left circumflex coronary artery were observed in 16 swines by coronary arteriography.The results showed significant improvement of myocardial function and significant high number of microvessel in intramyocardial transplantation animals compared with control group （P 〈 0.05）. [Conclusion] Transplantation of the bone marrow mononuclear cells to chronic ischemic myocardium could improve cardiac function and promote myocardial angiogenesis.

关 键 词：骨髓单个核细胞 细胞移植 血管密度 心脏功能 

分 类 号：R542.2[医药卫生—心血管疾病]