不同剂量血管紧张素-(1-7)对心力衰竭大鼠心室有效不应期及心室颤动阈值的影响  

Effects of Angiotensin-(1-7) at Different Doses on Ventricular Effective Refractory Period and Ventricular Fibrillation Threshold in Rats with Chronic Heart Failure

作　　者：方昶[1] 伍卫[1] 黄至斌[1] 邓义军[1] 刘英梅[1] 

机构地区：[1]中山大学附属第二医院心内科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2006年第3期297-301,共5页Journal of Sun Yat-Sen University:Medical Sciences

摘　　要：目的观察不同剂量血管紧张素-(1-7)对慢性心力衰竭大鼠心室有效不应期及心室颤动阈值的影响。方法40只Wistar大鼠成功建立慢性心力衰竭模型,随机分为心力衰竭组(n=10)、心力衰竭并血管紧张素-(1-7)处理3个剂量组(每组n=10)。另取10只Wistar大鼠行假手术,为正常对照组。用心电生理研究方法测定心室有效不应期(VERP)、心室颤动阈值(VFT)的变化,比较心力衰竭并血管紧张素-(1-7)处理的3个剂量组、心力衰竭组及正常对照组各项指标。结果心力衰竭大鼠VERP(73.5ms±2.4ms)较正常对照VERP(60.0ms±4.1ms)延长,VFT(6.7mA±1.4mA)较正常对照(30.4mA±1.3mA)降低。血管紧张素-(1-7)5!g.kg-1处理心力衰竭大鼠VERP及VFT较心力衰竭大鼠无显著性差异(P>0.05)。血管紧张素-(1-7)15!g.kg-1处理心力衰竭大鼠VERP(63.0ms±2.6ms)较心力衰竭大鼠VERP(73.5ms±2.4ms)缩短,VFT(17.1mA±3.1mA)较心力衰竭大鼠(6.7mA±1.4mA)增高。血管紧张素-(1-7)25!g.kg-1处理心力衰竭大鼠VERP(62.5ms±2.6ms)较心力衰竭大鼠VERP(73.5ms±2.4ms)缩短,VFT(19.0mA±2.4mA)较心力衰竭大鼠(6.7mA±1.4mA)增高,且较血管紧张素-(1-7)15!g.kg-1处理心力衰竭大鼠VFT(17.1mA±3.1mA)增高。相关分析显示:VERP与VFT负相关;血管紧张素-(1-7)的3个不同处理剂量与VERP负相关,与VFT正相关。结论血管紧张素-(1-7)能改善心力衰竭大鼠心电生理不稳定状态,提高心室颤动阈值,并具有剂量效应关系。[Objective] The research was aimed to investigate the effects of angiotensin-（1-7） at different doses on ventricular effective refractory period and ventricular fibrillation threshold in rats with chronic heart failure. [Methods] Chronic heart failure models in Wistar rats were set up. Forty rats with chronic heart failure were divided randomly into the heart failure group （n=10）, the heart failure with angiotensin-（1-7） 5 μg·k^-1 treatment group （n= 10）, the heart failure with angiotensin-（1-7） 15μg·k^-1treatment group （n=10） and the heart failure with angiotensin-（1-7） 25μg·k^-1 treatment group （n=10）. The control group included 10 sharn-operatod rats. The changes of cardiac electrophysiological parameters including ventricular effective refractory period （VERP） and ventricular fibrillation threshold （VFT） were assessed and compared among heart failure with angiotensin-（1-7） treatment groups, heart failure group, and control group. [Results] Compared with control rats, VERP in rats with chronic heart failure was prolonged （73.5 ms±2.4 ms vs 60.0 ms±4.1 ms） and VFT decreased （6.7 mA±l.4 mAvs 30.4 mA±l.3 mA ）. Angiotensin-（1-7） at a dose of 5μg·k^-1 did not change VERP and VFT in rats with chronic heart failure. Angiotensin-（1-7） at a dose of 15μg·k^-1 shortened VERP （from 73.5 ms=L2.4 ms to 63.0 ms±2.6 ms） and increased VFT （from 6.7 mA±l.4 mA to 17.1 mA±3.1 mA） in rats with chronic heart failure. Angiotensin-（1-7） at a dose of 25μg·k^-1 shortened VERP （from 73.5 ms±2.4 ms to 62.5 ms±2.6 ms） and increased VFT （from 6.7 mA±l.4 mA to 19.0 mA±2.4mA）in rats with chronic heart failure.Compared with angiotensin-（1-7） at a dose of 15μg·kg^-1,Angiotensin-（1-7）at a dose of 25μg·kg^-1 inereased VFT（from 17.1 mA±3.1mA to 19.0 mA±2.4mA）.VERP had negative correlation with VFT.The three doses of angiotensin-（1-7）had negative correlation with VERP and positive correlation with VFT.[Conelusion]Angiotensin

关 键 词：血管紧张素-(1-7) 心力衰竭 心室有效不应期 心室颤动阈值 

分 类 号：R546.1[医药卫生—心血管疾病]