细菌感染对微波行兔保脾术后NF-_κBp65表达的影响  

Influence of bacterial infection on expression of nuclear factor-kappa B p65 after spleen-preservation operation in rabbits using microwave coagulation

作　　者：鞠新华[1] 张宇[1] 姜伟国[2] 夏振龙[1] 

机构地区：[1]中国医科大学附属第二医院普外科,辽宁省沈阳市110004 [2]中国医科大学附属第二医院病理科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2006年第10期1001-1003,共3页World Chinese Journal of Digestology

摘　　要：目的:探讨细菌感染在微波行脾部分切除手术后NF-κBp65表达影响及意义.方法:日本大耳白兔随机分为两组,假拟手术组6只与实验组12只,即微波行脾部分切除手术组.手术后1mo耳静脉注射细菌悬浮液,观察48h动物的生存,测定残留脾脏质量和NF-κBp65表达.结果:注射细菌48h内动物均无死亡.两组间脾脏质量无显著性差异(P>0.01).镜下见脾脏残端固化处纤维化.NF-κBp65主要分布在胞浆或胞核内呈棕黄色.实验组NF-κBp65表达计数指数与假似手术组比较无显著性差异(P>0.01).结论:微波对脾脏损伤在短期内可修复,微波行保脾手术有一定价值.AIM： To investigate influence and significance of bacterial infection on the expression of nuclear factor-kappa B p65 （NF-κBp65） after partial splenectomy of rabbits using microwave coagulation. METHODS： Japanese white rabbits with large ears were randomly divided into sham operation group （n = 6） and partial splenectomy group （n = 12）. Microwave coagulation was adopted in the latter. Streptococcus pneumonia suspension was injected into ear veins 1 mo after operation in both groups, and within 48 h, the survival of the animals was observed. The spleen samples were weighted and the expression of NF-κBp65 in the residual spleen was detected. RESULTS： No animals died within 48 hours after bacterial injection. The qualities of the residual spleen were not significantly different between the two groups. Under microscope, fibrosis was showed in the remnant end of spleen coagulated by microwave. NF-κBp65 was mainly distributed in the cytoplasm or nucleolus. There was no significance difference between the twos groups in NF-κBp65 expression. CONCLUSION： Splenic injury coagulated by microwave can be restored in short term. Spleenpreservation operation using microwave coagulation is valuable in clinical practice.

关 键 词：微波 脾部分切除术 细菌感染 NF-ΚBP65 兔 

分 类 号：R657.6[医药卫生—外科学]