平阳霉素对源于人静脉畸形的内皮细胞增殖和黏附分子表达的影响  

Effects of pingyangmycin on the proliferation and expressions of adhesion molecules of human venous malformations endothelial cells

作　　者：司亚萌[1] 贾俊[2] 贾玉林[1] 熊学鹏[1] 赵怡芳[2] 

机构地区：[1]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,湖北武汉430079 [2]武汉大学口腔医学院口腔颌面外科,湖北武汉430079

出　　处：《中国口腔颌面外科杂志》2006年第3期220-224,共5页China Journal of Oral and Maxillofacial Surgery

摘　　要：目的:观察平阳霉素对体外培养的源于人静脉畸形的内皮细胞(humanvenousmalformationsendothelialcells,HVMECs)增殖和黏附分子表达的影响。方法:采用MTT法检测不同质量浓度平阳霉素(0.01～100μg/ml)作用不同时间(1、6、12、24h)后对HVMECs的增殖抑制率,利用细胞免疫化学及细胞ELISA法检测不同质量浓度平阳霉素作用不同时间后HVMECs黏附分子的表达。采用SPSS11.0统计软件包进行单因素方差分析。结果:平阳霉素的增殖抑制效应表现为剂量依赖性和时间依赖性。未受刺激的HVMECs不表达E-selectin(CD62E)及vascularcelladhesionmolecule-1(CD106)。10μg/ml及100μg/ml平阳霉素明显抑制HVMECs的增殖,未诱导出CD62E及CD106的表达。0.1～1μg/ml平阳霉素诱导HVMECs表达CD62E,而0.5～1μg/ml平阳霉素诱导出CD106的表达,此质量浓度对HVMECs增殖的抑制不明显。结论:平阳霉素增殖抑制表现出剂量和时间依赖效应。低质量浓度平阳霉素不明显抑制HVMECs的增殖,诱导出CD62E和CD106的表达,可能在平阳霉素治疗静脉畸形所引发的炎症反应中起着重要作用。PURPOSE： To study the effects of pingyangmycin （PYM） on the proliferation and expressions of adhesion molecules of human venous malformations endothelial cells （HVMECs）. METHODS： The proliferation inhibition of PYM on HVMECs was measured by MTT assay. Expressions of adhesion molecules were investigated by immunocytochemistry and cellular ELISA. SPSS 11.0 software package was used for one way ANOVA. RESULTS： The inhibition rates showed a dose-dependent and time-dependent effect. In the control group, no expressions of E-selectin （CD62E） and vascular cell adhesion molecule-1 （CD106） were observed in HVMECs. 10μ g/ml and 100μg/ml PYM, which didn＇t induce expressions of CD62E and CD106, had significant inhibitory effects on HVMECs. In contrast, increased expression of CD62E was detected after HVMECs were exposed to 0.1-1μg,/ml PYM and expression of CD106 was detected after HVMECs were exposed to 0.5-1μg/ml PYM. CONCLUSIONS： The inhibitory effects of PYM on HVMECs has a dosedependent and time-dependent relation. Low concentrations of PYM have a mild inhibitory effect on cellular proliferation but can stimulate HVMECs to express CD62E and CD106, which may play some role in the inflammatory process of venous malformations treated by PYM.

关 键 词：平阳霉素 静脉畸形 内皮细胞 增殖 黏附分子 

分 类 号：R965[医药卫生—药理学]