静脉免疫球蛋白对新生儿脐血淋巴细胞TGF-β1和IL-10分泌的影响  

Effects of intravenous immunoglobulin on IL-10 and TGF-β1 production in neonatal umbilical cord blood lymphocytes

作　　者：赵惠君[1] 郝轶群[1] 陈同辛[1] 朱亚忠[1] 李庆生[1] 

机构地区：[1]上海交通大学医学院附属新华医院儿内科上海市儿科医学研究所免疫/肿瘤研究室,上海200092

出　　处：《临床儿科杂志》2006年第5期378-381,共4页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(编号:39600158)

摘　　要：目的从IL-10和TGF-β1分泌的角度研究IVIG对新生儿淋巴细胞免疫功能的调节作用。方法应用IVIG和PHA不同组合对脐带血单个核细胞(cord blood mononuclear cell,CBMC)或CD3+T淋巴细胞进行刺激培养;应用ELISA法检测细胞IL-10和TGF-β1的分泌,并与成人外周血单个核细胞比较。结果IVIG可抑制PHA诱导的新生儿CBMC IL-10和TGF-β1的分泌。IVIG对新生儿CBMC IL-10和TGF-β1分泌的抑制作用与细胞是否被活化无关。IVIG对脐带血CD3+T淋巴细胞IL-10分泌的抑制作用明显低于新生儿CBMC,对脐带血CD3+T淋巴细胞TGF-β1分泌无明显影响。结论IVIG对淋巴细胞的免疫抑制并非通过诱导抑制性细胞因子分泌发挥作用,可能通过直接作用于淋巴细胞或通过其他间接机制而起作用。IVIG对IL-10和TGF-β1分泌的影响可能因所作用的细胞类型和细胞所处的状态不同而不同,因此应在充分了解新生儿免疫状态的基础上合理使用IVIG。Objective To explore the mechanism of immunosuppressive effects of intravenous immunoglobulin （IVIG） on neonatal immune function with the aid of IL-10 and TGF-β1 produced by umbilical cord blood mononuclear cells （CBMCs） and CD3^＋ T lymphocytes. Methods Umbilical cord blood mononuclear cells （CBMCs） and CD3^＋ T lymphocytes were isolated from 8 neonates. IL-10 and TGF-β1 produced by CBMCs or CD3^＋ T lymphocytes with various stimuli in different combinations of IVIG and phytohemagglutinin （PHA） were measured with ELISA and compared with those produced by peripheral blood mononuclear cells from 8 adults （PBMCs）. Results IVIG obviously reduced IL-10 and TGF-β1 production of CBMCs after PHA stimulation （P〈 0.01）. This inhibition effect was independent of whether CBMCs activated with PHA or not. IVIG could also inhibit IL-10 production of cord blood CD3^＋T lymphocyte, but the degree of inhibition was much lower than that produced by CMBCs with no significant effect on TGF-β1 production of cord blood CD3^＋ T lymphocyte. Conclusions IVIG-mediated inhibition effects on IL-10 and TGF-β1 produced by lymphocytes may be derived from some direct effects on lymphocytes or other indirect mechanisms rather than from production of suppressive cytokines. The inhibition effects of IVIG on IL-10 and TGF-β1 production depends on the types and characteristics of the cells that IVIG acted on indicating that IVIG should be used properly on the base of knowing well about the immunological characteristics of neonates.

关 键 词：新生儿 淋巴细胞 静脉免疫球蛋白 细胞因子 免疫抑制 

分 类 号：R722.1[医药卫生—儿科]