ISSR和RAPD-PCR技术对东北地区主推玉米品种的鉴别  

Indentification of Varieties among Eitheen Northeast Dominat Maize by ISSR and RAPD-PCR Methods

作　　者：李尚禹 王春艳[2] 张朝燕[3] 李校堃[4] 

机构地区：[1]长春市产品质量监督检验院,长春130012 [2]吉林大学公共卫生学院,长春130021 [3]长春师范学院生物系,长春130032 [4]吉林农业大学生物工程技术研究院

出　　处：《吉林大学学报（理学版）》2006年第3期509-512,共4页Journal of Jilin University:Science Edition

基　　金：国家高技术研究发展计划项目基金(批准号:2001AA215131).

摘　　要：运用现代分子生物学简单重复区间序列扩增多态性(ISSR)和随机扩增片段多态性DNA(RAPD)两种分子标记技术,对吉林省主推6个玉米杂交种及其父本、母本共计18份基因图谱进行鉴别.以ISSR为主要检测方法、RAPD为验证方法,分别从30条ISSR引物中选出4条、从30条RAPD引物中筛选出2条扩增效果明显、特异性高的引物.结果表明,运用ISSR和RAPD-PCR技术提高了玉米品种鉴定和纯度鉴定的准确性.The gene maps of eighteen maize samples were analyzed by inter-simple sequence repeat （ISSR） and random amplified polymorphic DNA （RAPD） marker methods. The ISSR method was the primary method and was validated by the RAPD method. 4 highly polymorphic primers were screened out from 30 random ISSR primers and 2 highly polymorphic primers were screened out from 30 random RAPD primers. In conclusion, uniting ISSR combined with RAPD can raise the veracity of indentification.

关 键 词：玉米 ISSR RAPD 品种鉴别 

分 类 号：S513.033[农业科学—作物学] O789[理学—晶体学]