血清纤维结合蛋白的酶免疫检测法  

Study of enzyme immunoassay method for measuring fibronectin in serum

作　　者：张超[1] 

机构地区：[1]安徽理工大学医学院生物化学与分子生物学教研室,淮南232001

出　　处：《贵州医药》2006年第5期414-416,共3页Guizhou Medical Journal

摘　　要：目的建立血清纤维结合蛋白(Fn)含量的酶免疫分析(EIA)检测方法。方法利用现有Fn检测试剂盒建立Fn的EIA测定法,并对所建立的方法从灵敏度、精密度、特异性、重复性等方面进行方法学评价。结果该法灵敏度为11．69ml／L,线性范围为50～300mg／L,批内变异系数为 3．82％,批间变异系数为5．10％,回收率97．7％。肺结核组及正常对照组血清Fn含量分别为 (188．82±5．51)mg／L和(275．25±18．11)mg／L,两组间差异有显著性意义(P<0．01);肺结核稳定期患者血清Fn显著高于进展期及好转期(P<0．05)。结论该方法线性范围适宜,准确度、精密度较好,符合临床检验工作要求。Objective To develop an enzyme immunoassay（EIA） method suitable for measuring fibronectin（Fn） concentration. Methods The EIA method was established with test and measurement of Fn kit. The methodology including its sensitivity, precision, recovery and specificity was evaluated. Results he assay provided a sensitivity of 11.69mg/L and line range was 50 to 300mg/L. The average within-run and between-run coefficients of variation（CV） were 3.82% and 5. 10% respectively. The average recovery rate of Fn was 97. 7%, the serum Fn values in pulmonary tuberculosis（TB） group and normal controls group were （188. 82±5. 51）mg/L and（275. 25±18.11）mg/L（P〈0. 01）. The serum Fn level of the patients in stable stage was significandy higher than that of active stage and that of restoration stage（P〈0. 05）. Conclusion The line range of this method was temperate, the accuracy and precision of this method were all higher and coincided with the regard of clinical testing.

关 键 词：纤维结合蛋白 酶免疫分析法 方法学 评价研究 

分 类 号：R446.11[医药卫生—诊断学]