超抗原SEA对两种小鼠脾脏淋巴细胞的不同活化作用的探讨  

Studies on the Proliferative activation effect of mice spleen lymphocytes by superantigen SEA

作　　者：余德厚[1] 陆洪光[1] 程波[2] 吴承龙[3] 汪宇[1] 牟霞[1] 

机构地区：[1]贵阳医学院附属医院皮肤科,550004 [2]福建医科大学附属医院皮肤科 [3]贵阳医学院微生物学教研室,550004

出　　处：《贵州医药》2006年第4期295-299,共5页Guizhou Medical Journal

基　　金：本课题受国家自然基金项目资助(D-39960071)

摘　　要：目的研究超抗原SEA(葡萄球菌肠毒素A)对C57BL／6小鼠和昆明种小鼠脾脏淋巴细胞的不同活化作用。方法淋巴细胞转化实验采用MTT法;细胞因子IL-2检测采用酶联免疫吸附测定法(双抗夹心法)。结果超抗原SEA对C57BL／6小鼠和昆明种小鼠脾脏淋巴细胞均有明显的促增殖作用,但对C57BL／6小鼠脾脏淋巴细胞活化作用强于对昆明种小鼠。结论超抗原SEA对体外小鼠脾脏淋巴细胞的促增殖作用与其浓度、刺激时间及小鼠的种类有关。Objective To investigate the proliferative activation of mice spleen lymphocytes in response to superantigen SEA. Methods MTT assay were used to detect the proliferation of mice spleen lymphocytes and ELISA to detect IL-2 levels produced by the activated lymphocytes. Results Both the CATBL/6 and Kunming mice spleen lymphocytes can be activated by superantigen SEA. The proliferative activation of lymphocytes in CATBL/6 mice spleen with SEA was greater than Kunming mice. IL- 2 production arrived at top level during the period of 4872h. Conclusion There are correlation between the spleen lymphocytes activation and SEA stimulation concentration.

关 键 词：超抗原 葡萄球菌肠毒素A 淋巴细胞转化 

分 类 号：R378.11[医药卫生—病原生物学] R329.28[医药卫生—基础医学]