复肝灵对兔四氯化碳肝损伤后的影响  

Experiment study on the effect of Fuganling on liver injury induced by CCl_4 in rabbit

作　　者：巩念明[1] 魏璐婉[1] 孙平[1] 毕玉顺[1] 刘执玉[1] 

机构地区：[1]山东大学医学院淋巴学研究室,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第5期459-462,466,共5页Journal of Shandong University:Health Sciences

基　　金：国家科委重点攻关课题(029010572)

摘　　要：目的:观察中药制剂复肝灵颗粒对兔CCl4肝损伤的治疗作用。方法:采用10%CCl4油溶液5 ml/kg皮下注射建立兔实验性肝损伤模型,设生理盐水对照组、CCl4模型组、复肝灵(FGL)高、中、低剂量治疗组,对血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、r-谷氨酰基转移酶(r-GT)、总蛋白、白蛋白、球蛋白进行检测研究。结果:CCl4模型组体重明显降低,与生理盐水对照组相比有显著的统计学意义(P<0.01),FGL治疗组体重有所降低,但与生理盐水对照组相比无统计学意义(P>0.05);CCl4模型组兔血清AST、ALT和r-GT较生理盐水对照组和3个FGL治疗组显著增加(P<0.01);FGL 3个剂量组均能明显降低CCl4所引起的肝组织丙二醛(MDA)的升高(P<0.01);在光学和电子显微镜下显示的组织学结构,FGL治疗组与对照组相比均有明显的修复。结论:复肝灵对兔CCl4肝损伤有良好的保护作用。Objective： To study the effect of traditional Chinese medicine ＂Fuganling＂ （FGL）on liver injury induced by CC14 in rabbits. Methods： Experimental liver injury models of rabbits were established by hyodermic injection of CC14 oil solution（ 10%, 5 ml/kg）, and then they were distributed to pumping brine group（control）, CC14 group, and high, median, low dose FGL group. The blood serum aspartate aminotransferase （AST）, the glutamicpyruvic transaminase（ALT）, the r-glutamltransferase（r-GT）, the gross protein, the albumin, and the globin were tested 2 months later. Results： （1） The body weights decreased obviously in CCl4-indued injury group compared with brine group（ P 〈 0.01 ）, while they decreased unconspicuously in FGL therapy groups compared with brine groups （P 〉 0.05）; （2） levels of serum AST, ALT and r-GT in CCl4-induced injury groups increased obviously compared with FGL therapy groups （P 〈 0.01）;（3） CCl4-indued liver MDA enhance was decreased by FGL evidently （P 〈 0.01 ）;（4） Microscope and electron microscopy revealed that the liver structure had been ameliorated in Fuganling treatment groups. Conclusion： Fuganling has obviously protection effect on liver injury.

关 键 词：四氯化碳 肝功能试验 兔 复肝灵颗粒 

分 类 号：R285[医药卫生—中药学] R286[医药卫生—中医学]