肠易激综合征大鼠模型外周血免疫调节性T细胞的研究  

Regulatory T cells in peripheral blood in rat model with post infectious irritable bowel syndrome

作　　者：郭敏[1] 李延青[1] 刘传勇[2] 谢冬萍[2] 郭玉婷[1] 左秀丽[1] 曾娟[1] 

机构地区：[1]山东大学齐鲁医院消化科,山东济南250012 [2]山东大学医学院生理学研究所,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第5期514-518,共5页Journal of Shandong University:Health Sciences

摘　　要：目的：观察外周血免疫调节性T细胞（Treg）在感染后肠易激综合征（PI-IBS）大鼠模型中的变化．探讨Treg在其发病机制中的作用。方法：随机将16只大鼠分为模型组和正常对照组，分别用福氏Ⅳ型志贺氏痢疾杆菌和生理盐水灌胃。模型组急性肠道感染16～22d，测定两组大鼠的肠道感觉阈值和平滑肌张力积分，远端结肠行病理学检查，用流式细胞仪分析外周血Treg的比例。结果：模型组肠道感觉阈值明显降低（P〈0．05），肠道平滑肌张力积分明显升高（P〈0．05），而组织学无明显变化，表明PI-IBS大鼠模型建立成功；模型组外周血Treg／CD4^＋T比值明显下降（P〈0．05）。结论：大鼠在急性肠道感染消失后．会出现PI-IBS；免疫调节的异常参与了PI-IBS的发病过程。Objective： To observe the changes of regulatory T cells （Treg ceils） in peripheral blood in rat model with post infectious irritable bowel syndrome （PI-IBS） and investigate the role of Treg cells in its pathogenesis. Methods： A total of 16 female rats were divided into model and control groups, who were given shigeila flexneri by gavage and saline by gavage, respectively. After the bowel inflammation had been induced for 16- 22 days, all rats were treated with colon-rectal balloon distention; the contraction of enteric smooth muscle were measured in vitro and the proportion of Treg ceils in peripheral blood was detected by using flow cytometry analysis. Results： In model group, the perceptual threshold decreased and the tension integral of enteric smooth muscle increased greatly compared with the resuits from control group（ P 〈 0.05）, while no obvious histological changes had been observed, suggesting rat model of PI-IBS had been successfully established; the value of Treg/CD4^＋ T in peripheral blood decreased significandy compared with the result from control group（ P 〈 0.05）. Conclusion： Bowel functional disorder is prolonged after acute enteric inflammation has disappeared. Abnormal immunoregulation takes account for the pathogenesis of PI-IBS.

关 键 词：结肠疾病 功能性 免疫调节性T细胞 感染 肠功能紊乱 

分 类 号：R392.32[医药卫生—免疫学]