白蜡虫抗突变实验与主要功效成分分析  

Anti-mutation Experiment of White Wax Scale(Ericerus pela) and Analysis of Main Function Factors

作　　者：冯颖[1] 陈晓鸣[1] 何钊[1] 郭宝华[1] 马艳[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明650224

出　　处：《林业科学研究》2006年第3期284-288,共5页Forest Research

基　　金：国家攻关项目(2004BA502B04);云南省科技攻关项目(2001NG16);科技部成果转化项目(02EFN215301189)部分研究内容

摘　　要：对白蜡虫的抗突变功能进行实验研究,并分析了甲壳素、多糖、维生素和黄酮类等。以白蜡虫为抗突变材料喂饲小鼠,48 h检测微核的发生率,各剂量组均比对照组的微核率低,其中高剂量组与对照有显著差异,抑制率为34.19%,说明白蜡虫能够对抗环磷酰胺产生的致突变作用,具有明显的抗突变功能。分析结果表明:白蜡虫含有2.14%甲壳素;7.2%的粗多糖,除蛋白后的多糖含量4.54%,多糖含量以葡萄糖计为28.4%,由葡萄糖、甘露糖和果糖组成,3种糖的摩尔比为5∶21∶1,白蜡虫多糖为一种杂多糖-蛋白复合物;白蜡虫还含有1.3%的总黄酮和多种维生素。甲壳素、多糖、维生素和黄酮类物质应为抗突变作用的主要功效成分。This paper dealt with the anti-mutation experiment of white wax scale and analysis of main functional factors. The micro-nucleoli rates in every experimental dose groups were lower then the contrast in 48 h. The difference between high dose group and contrast was obvious. The restriction rate of micro-nucleoli in high dose group was 34.19%. The results showed that white wax scale could restrict mutation caused by C. P and had clear anti-mutation function. White wax scale contained 2.14% chitin. White wax scale contained 7.2% crude polysaccharide. The polysaccharide content was 4. 54% after removing protein by enzyme. The sugar content of the polysaccharide was 22.1% as glucose, composed of glucose, galactose and fructose in molar ratio of 5： 21： 1. The polysaccharide isolating from white wax scale was polysaccharide-protein complex. This insect contained 1.3% flavonoid and several kinds of vitamins, chitin, polysaccharide, vitamin and flavonoid were main anti-mutation functional factors of white wax scale.

关 键 词：白蜡虫 抗突变 甲壳素 多糖 B族维生素 黄酮 

分 类 号：S899.1[农业科学—特种经济动物饲养]