侧柏挥发油成分及抗肿瘤活性的研究  

Volatile Constituents from Platycladus orentalis and Their Antitumor Activities

作　　者：蒋继宏[1] 李晓储[1] 高雪芹[1] 高甜惠[1] 陈凤美[1] 冯友建[1] 黄利斌[2] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点实验室,江苏徐州221000 [2]江苏省林业科学研究院,江苏南京211153

出　　处：《林业科学研究》2006年第3期311-315,共5页Forest Research

基　　金：国家"十五"科技攻关重大专项课题"中国城市森林网络体系‘点’的研究与示范"(2001BA516A15-07);国家林业局科技项目"城镇绿化乔木树及生态功能性树种区试"(2002-17A)协作研究内容之一;江苏省药用植物生物技术重点实验室开放课题(编号(02AXL12)研究内容

摘　　要：用A lam ar b lue法对提取的侧柏叶、种皮及种子挥发油进行了抗人肺癌细胞实验,结果表明侧柏叶、种皮和种子挥发油对肺癌细胞NC I-H460有明显抑制率,分别为86.24%、47.80%和97.73%。其中以叶挥发油和种子挥发油最高。采用气-质联用法对侧柏叶、种皮和种子挥发油的化学成分进行了研究,其含有的挥发物质种类侧柏叶、种皮和种子分别为8,31和21个成分,其挥发油成分也不尽相同,侧柏叶含量最高的是α,,α4,8-四甲基-3,7环癸二烯-1-甲醇和雪松醇分别占总挥发油的27.213%和26.193%,种皮和种子挥发油中含量最高的都是雪松醇,分别占总挥发油的28.277%和26.919%。其中,对侧柏叶挥发油4℃低温下重结晶得到的雪松醇进行了鉴定,纯度达97.06%,对雪松醇抗人肺癌细胞实验结果是雪松醇对人肺癌细胞NC I-H460半致死浓度为44.98μg.mL-1。The antitumor activities of volatile constituents from Platycladus orentalis against human lung cancer cell line NCI-H460 were determined by alamar blue assay. The results showed that the inhibiting rates of volatile constituents from the leaves, seed and seed capsule of P. orentalis against human lung cancer cell line NCI-H460 were 86.24% , 97.73% and 47.80% respectively. And the volatile oil constituents in leaves and seed were the highest. The volatile constituents from the three organs were analyzed by GC-MS method. The varieties of volatile oil in the leaf,seed and seed capsule of P. orentalis were 8,31 and 21 respectively. The results showed that the leaves contained the highest contents of -α, α ,4,8-tet- ramethy 1-3,7-cyclodecadiene-1-methjanol and cedrol,which took about 27. 213% and 26. 193% respectively. The contents of cedrol in seed and seed capsule were the highest,which were 28. 277% and 26. 919% of the total. The purity of cedrol re-crystallized under 4℃ from leaves of P. orentalis was as high as 97. 06%. The ICS0 of cedrol against the lung cancer cell line NCIoH460 was 44. 98μg·mL^-1.

关 键 词：侧柏 挥发油 雪松醇 抗肿瘤活性 

分 类 号：S791.38[农业科学—林木遗传育种] R965[农业科学—林学]