山地木麻黄菌根菌的筛选和接种效应的研究  

Mycorrhizal Fungal Screening and Inoculant Effectiveness for Casuarina junghuhniana

作　　者：张勇[1] 陈羽[1] 李国标 陈珍[1] 仲崇禄[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520 [2]广东省清远市林业局,广东清远511515

出　　处：《林业科学研究》2006年第3期342-346,共5页Forest Research

基　　金：863项目"生态环境建设抗逆林灌木柠条;沙棘等新品种选育"的子专题:"木麻黄抗逆新品种选育"(2002-2005);IFS项目"外生菌根菌抗木麻黄青枯病的研究"部分研究内容;广东省自然科学基金项目"利用菌根菌提高木麻黄抗旱性的研究"

摘　　要：利用3个内生菌根菌(AMF)和6个外生菌根菌(ECMF)接种山地木麻黄苗,测定小苗的树高、根长、地径、地上干质量、地下干质量和总生物量,并在干旱胁迫下测定小苗的保存率。结果表明:接种内、外生菌根菌后都能极显著地促进山地木麻黄苗期的生长;山地木麻黄对供试的AMF和ECMF菌根都属于中等强度或较弱的依赖性;山地木麻黄接种菌根菌后对地上部分(苗高、地径和地上干质量)生长的促进作用比地下部分(根长和地下干质量)要大;在供试的9个菌根菌种和菌株中,AMF比ECMF更能提高山地木麻黄的抗旱力;筛选出菌根效应较好的菌根菌有:苏格兰球囊霉90068、苏格兰球囊霉90036、地表球囊霉9004、黄硬皮马勃0207、蜡蘑E439,可在山地木麻黄苗期接种应用。The paper reports the preliminary results of inoculant effect on the growth and drought resistance of Casuarina junghuhniana based on a nursery experiment. Seedlings were inoculated with 3 endomycorrhizal fungi （AMF）and 6 ectomycorrhizal fungi （ ECMF）, then the seedling height, root length, ground diameter, dry weight under ground, dry weight above ground and total biomass were measured, and the survival percentage of seedlings under drought stress was surveyed after no watering for 7 days as well. The results showed that both fungi could significantly improve the growth of C. junghuhniana seedlings. Mycorrhizal Dependency （MD） of C. junghuhniana belongs to medium or weak in classification according to 9 fungi applied in this experiment. The growth improvement of above ground （ such as seedling height, diameter at ground level and dry weight above ground） was more evident than that of under ground （ such as root length, dry weight under ground） when C. junghuhniana was inoculated with mycorrhizal fungi. The AMF indicated more obvious effect in improving drought resistance of C. junghuhniana than that of ECMF according to 9 mycorrhizal fungi used in this experiment. 5 isolates were selected from tested AMF and ECMF, including Glomus caledonium 90068, G. caledonium 90036, G. versiform 9004, Scleroderma flavidum 0207, Laccaria sp E439. They could be used as inoculant of C. junghuhniana seedlings.

关 键 词：山地木麻黄 菌根菌 菌根依赖性 接种效应 

分 类 号：S792.93[农业科学—林木遗传育种]