柑橘提取物诺必擂停抑制肝癌增殖的免疫学机制  

Immunological Mechanisms of Nobiletin,a Citrus Polymethoxy Flavonoid in Inhibiting Hepatocarcinoma Proliferation

作　　者：成美英[1] 葛慧[1] 张洪泉[1] 

机构地区：[1]扬州大学医药研究所,江苏扬州225001

出　　处：《中药新药与临床药理》2006年第3期161-164,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的研究诺必擂停(NOB)对小鼠肝癌移植瘤Heps的作用及其免疫学机制。方法建立小鼠肝癌移植瘤Heps实体型模型,解剖剥离肿瘤称重,计算抑瘤率;通过迟发性超敏反应观察整体细胞免疫机能;用MTT法检测T、B淋巴细胞增殖反应;乳酸脱氢酶(LDH)释放法检测CTL、NK细胞杀伤活性。结果NOB灌胃给药对小鼠肝癌Heps有明显抑制作用;显著提高了荷瘤小鼠降低的迟发性超敏反应;荷瘤小鼠T、B淋巴细胞增殖反应明显提高;同时CTL、NK细胞杀伤活性显著增强。结论提高机体免疫功能可能是NOB抗小鼠肝癌移植瘤Heps增殖的一个重要途径。Objective To investigate the effect of nobiletin, a citrus polymethoxy flavonoid, on hepatocarcinoma Heps in mice and to explore its immunological mechanism. Methods Mice tumor models were made by transplantation of Heps hepatocarcinoma cell strain. The tumor mass was weighed to calculate the tumor - inhibitory rates. The whole cellular immunity was observed by delayed type hypersensitivity （DTH）. MTT assay was used to detect the proliferation of T and B lymphocyte, and the activities of cytotoxic T lymphocytes （CTL） and natural killing cells （NKC） were measured by lactate dehydrogenase （LDH） releasing method. Results Nobiletin could markedly inhibit the growth of hepatocarcinoma Heps （solid tumor）. The decreased DTH in hepatocaricinoma mice was enhanced significantly, and T and B lymphocyte proliferation were increased by nobiletin. Meanwhile, the killing activities of CTL and NKC were promoted obviously. Conclusion Improvement of the immune function may be a vital route of nobiletin in inhibiting the proliferation of hepatocarcinoma Heps in mice.

关 键 词：诺必擂停 肝癌 细胞增殖 免疫学 机制 

分 类 号：R285.5[医药卫生—中药学]