对硝基苯甲酸(PNB)替代BACTEC460NAP进行分支杆菌分群的初步研究  

Study on Identification of Mycobacterium Tuberculosis Complex and Atypical Mycobacteria using PNB instead of NAP in BACTEC System

作　　者：刘晓雪[1] 丁北川[1] 安燕生[1] 

机构地区：[1]北京结核病控制研究所,100035

出　　处：《中国防痨杂志》1996年第1期23-25,共3页Chinese Journal of Antituberculosis

摘　　要：用PNB替代BACTEC系统中NAP药物,将PNB加入7H_(12)B培基内,鉴定结核菌与非结核分支杆菌两大菌群。实验10株标准菌株和115株临床菌株;结果:人、牛型标准菌株在PNB12B10μg/ml生长抑制。8株非结核标准菌株抑菌浓度PNB>500μg/ml。以PNB100μg/ml12B为界限,115株临床菌株PNB与NAP方法平行对照实验,鉴定结果:结核菌群符合率97.8％,非结核菌群符合率95.8％,总符合率97.4％(P>O.05)。其中18株用PNB法间隔一个月重复实验,两次结果一致。结果显示,PNB12B菌群鉴定方法可以做为一种准确、经济的菌型初筛实用方法。The identification of M. tuberculosis complex and atypical mycobacteria were studied by substituting NAP with PNB into the 7H12B vial in BACTEC system. Ten standard strains and 115 clinical strains were tested and analysed. The results showed that the growth of H37Rv and Bovis standard strains were inhibited at PNB 12B 10 μg/ml. Eight standard strains of atypical mycobacterial were inhibited at a concentration of PNB greater than 500 jig/ml. Compared with the method of NAP, a criterion of PNB 100 fig/ml was used to test the 115 clinical strains. The conformity rate of M. TB complex is 97. 8% , and that of atypical mycobacteria is 95. 8% , And the overall conformity is 97. 4% , (P>0. 05). Eighteen of the above 115 strains were retested by the PNB method after a month, with similar results. The results showed that the PNB 12B method can be considered as an accurate and economic method for primary identification of M. tuberculosis complex and atypical mycobacteria.

关 键 词：分支杆菌属 硝基苯甲酸 鉴定 结核杆菌 

分 类 号：R378.91[医药卫生—病原生物学]