用原位核酸杂交法探讨慢型克山病与柯萨奇B组病毒的关系  

Relationship between Coxsackie Virus B and Keshan disease hy hybridization in situ chronic Keshan disease in Shandong province

作　　者：钟学宽[1] 邹宁[1] 周令望[1] 刘艺[1] 漆炜[1] 付桂玲[1] 王爱云[1] 于维汉[1] 牛美娟[2] 曲章义[2] 郭彩玲[2] 谷鸿喜[2] 杨晓霞[3] 房维堂[3] 钟翔宇[4] 

机构地区：[1]哈尔滨医科大学克山病研究所,150086 [2]哈尔滨医科大学微生物教研室 [3]山东省地方病防治研究所 [4]哈医大

出　　处：《中国地方病学杂志》1996年第1期8-9,共2页Chinese Jouranl of Endemiology

摘　　要：为探讨山东慢型克山病与柯萨奇 B 组病毒的关系,用缺口平移法制备生物素标记 CoxB_3病毒的 cDNA 探针与慢型克山病14例尸检心肌组织及10例脑外伤,CO 中毒等意外死亡的正常成人心肌组织及10例非病区7～8个月引产胎儿的心肌组织切片进行原位杂交,14例慢克中有9例检测到柯萨奇 B 组病毒的 RNA,阳性率达64%,而对照组均为阴性。提示柯萨奇 B 组病毒感染可能是山东慢型克山病发病因素之一。We performed hybridization in situ to examine RNA of Coxsackie Virus B(CVB)in paroffin-em- bedded myocardial specimens from 14 patients with chronic Keshan disease,10 patients with cerebral trauma or carbon monoxide poisoning and 10 foetus aged 7～8 monthes from non endemic areas using the CVB_3 cDNA probe labelled with Bio-11duTP by nick translation.Of the 14 patients with chronic Ke- shan disease,9(64%)showed positive findings.Their myocardial tissues were infected by CVB previous- ly.The results suggested that CVB infection may be one of the major cause of chronic Keshan disease in Shandong province.

关 键 词：柯萨奇B组病毒 克山病 原位核酸杂交 

分 类 号：R542.302[医药卫生—心血管疾病]