HPLC法测定刺五加中两种三萜酸成分的含量  

Determination of Two Triterpene Acids in Acantopanax Senticosus (Rupr.et Maxim.) Harms by HPLC

作　　者：邹盛勤[1] 黄玉伦[2] 

机构地区：[1]宜春学院生物工程研究所,宜春336000 [2]宜春学院生物工程系

出　　处：《浙江中医药大学学报》2006年第3期290-292,共3页Journal of Zhejiang Chinese Medical University

基　　金：国家"863"计划重点资助项目(No:2002AA2Z3217)~~

摘　　要：[目的]采用超声技术提取中药刺五加中的乌索酸和齐墩果酸，并建立HPLC-PAD法测定乌索酸和齐墩果酸的含量。[方法]色谱拄为Nova-PakCl8（3．9×300mm，4μm），流动相甲醇-水（88：12），流速为0．8mL／min，二极管阵列检测器检测，检测波长210nm，拄温25℃。[结果]乌索酸线性范围：0．136～1．224μg，线性回归系数0．9995；齐墩果酸线性范围：0．052～0．468μg，线性回归系数0．9944。样品中乌索酸的平均回收率为98．1％，RSD为1.1％（n=5）{齐墩果酸的平均回收率为97．5％，RSD为1.0％（n=5）。[结论]HPLC法测定含量准确、可靠，可用于刺五加中乌索酸和齐墩果酸含量的测定分析。[Objective] Ursolic acid and oleanolic acid in Acantopanax senticosus （Rupr. et Maxim. ） Harms were extracted with the ultrasonic technology. A determination method of ursolic acid and oleanolic acid was established with the high performance liquid chromatograph and photodiode array detector.[Method]The chromatographic column, Nova-Pak C18 （3.9 × 300 mm, 4 bμm）, methanol-water （88 ： 12,V/V）, mobile phase with 0.8 mL/min flow rate, the detected wave length （210nm）,and the col umn temperature（25 ℃） were adopted.[Results] The calibration curve of ursolic acid was linear under the content of 0. 136-1. 224 μg and the correlation coefficient was 0. 9995. The calibration curve of oleanolic acid was linear under the content of 0. 052 -0. 468 μg and the correlation coefficient was 0. 9994. The average recovery of ursolic acid was 98. 1 % and RSD was 1. 1 % （n =5）. The average recovery of oleanolic acid was 97.5 % and RSD was 1.0 % （n=5）. [Conclusion]The content determination of HPLC is accurate and reliable, and can be used to analyse quantificationally ursolic acid and oleanolic acid in Acantopanax senticosus （Rupr. et Maxim. ） Harms.

关 键 词：刺五加 乌索酸 齐墩果酸 高效液相色谱法 含量分析 

分 类 号：R284.1[医药卫生—中药学]