强化SOD刺梨汁的抗突变作用  

Antimutagenic effect of Cili juice enriched with SOD

作　　者：张爱华[1] 龙曼海[1] 蒋宪瑶[1] 朱延韦 

机构地区：[1]贵阳医学院卫生系毒理研究室

出　　处：《中国药学杂志》1996年第3期144-147,共4页Chinese Pharmaceutical Journal

摘　　要：目的：强化ＳＯＤ刺梨汁（ＣＬＪＥＳ）为抗衰老保健药，研究其抗突变作用，以寻求更广阔的使用范围。方法：Ａｍｅｓ试验［鼠伤寒沙门杆菌回复突变试验］参照Ａｍｅｓ等１９８３年的方法，选用试验菌株ＴＡ９８，ＴＡ１００及阳性物（２－ＡＦ，ＡＦＢ１，ＮａＮ３）进行平板掺入试验。小鼠骨髓细胞微核（ＭＮ）和染色体畸变（ＣＡ）试验中，ＣＬＪＥＳ［４，８，ｌ６ｍｌ／（ｋｇ．ｄ）］经ｉｇ，连续５ｄ，未两次同时ｉｐ环磷酰胺［ＣＰ３０ｍｇ／（ｋｇ·ｄ）］，所有动物未次给药后处死。结果：ＣＬＪＥＳ５个剂量组（０．１，１，５，１０，１００μｌ／皿）显著抑制２－ＡＦ，ＡＦＢ１诱导的ＴＡ９８，ＴＡ１００（＋Ｓ９）和ＮａＮ３诱导的ＴＡ１００（－Ｓ９）的回复突变数，各剂量组间呈明显的剂量效应关系。ＣＬＪＥＳ明显降低ＣＰ诱发的小鼠ＭＮ率和ＣＡ率，亦有良好的剂量效应关系。结论：ＣＬＪＥＳ具有抗突变作用以及对遗传物质损伤的保护作用。To research the antimutagenic effect of Cili juice enriched with SOD(CLJES)and to seek its more widely applied range. METHODS:Ames assay(Salmonella typhimurium reverse mutation assay)was on the basis of the method of Ames et al(1983).The tester trains TA98 ,TA100 and three positive medicines(2-AF,AFB1,NaN3)were applied in the procedure of the standard plate incorporation.The CLJES[4,8,16 ml/(kg· d)]was administered in the chromosomal aberration(CA)test and the micronucleus(MN)test in mouse bone-marrow cell for 5 days.The positive medicine[cyclophosphimide,CP,30 mg/(kg·d)] was administered twice by ip after the last inoculation,then all mice were killed.RESULTS:Five dose groups(0.1,1,5,10,100μl/plate)in Ames assay obviously inhibited the number of revertant colonies of the tester strains TA98, TA100 induced by 2-AF,AFB1(with S9).The number of revertant colonies of the tester strains TA100 induced by NaN3(without S9)remarkably decreased too. There was significant correlation between the doses and the numbers of revertant colonies of the tester strains. It was found that CLJES[4,8,16 ml/(kg·d)] remarkably decreased the MN and CA rates in mice induced by CP. There was significant correlation between the three dose groups and the MN and CA rates.CONCLUSION:The CLJES had antimutagenic effect and could protect hereditary materials from being damaged.

关 键 词：强化SOD刺梨汁 AMES试验 抗突变作用 抗衰老药 

分 类 号：R977.9[医药卫生—药品]