瓦布贝母生物碱的分离与鉴定  

Isolation and Structure Flucidation of Alkaloids from the Bulb of Fritillaria Wabuensis S.Y. Tang et S.C. Yueh

作　　者：王化远[1] 张安将 唐心曜 郑永[1] 易小辉 郁开北 

机构地区：[1]成都药学院有机化学教研室,成都药学院中心测试室,成都药学院生药学教研室,重庆医科大学附二院,中科院成都分院晶体X-衍射室

出　　处：《华西医科大学学报》1996年第1期100-105,共6页Journal of West China University of Medical Sciences

摘　　要：从四川产家种贝母新种──瓦布贝母（ＢｕｌｂｕｓＦｒｉｔｉｌｌａｒｉａＷａｂｕｅｎｓｉｓＳ．Ｙ．ＴａｎｇｅｔＳ．Ｃ．Ｙｕｅｈ）鳞茎中分离出８种生物碱。经波谱分析，高效液相色谱，ＴＬＣ与对照品进行对照鉴定，确认碱Ⅰ为西贝索（ｉｍｐｅｒｉａｌｉｎｅ），碱Ⅱ为贝母辛（ｐｅｉｍｉｓｉｎｅ），碱Ⅱ为鄂贝乙素（ｅｂｅｉｎｏｎｅ），碱Ⅳ为异浙贝甲素（ｉｓｏｖｅｒｔｉｃｉｎｅ），碱Ⅴ为西贝素－β氮氧化物（ｉｍｐｅｒｉａｌｉｎｅ－β－Ｎ－ｏｘｉｄｅ）。作者对碱Ⅴ的１Ｈ，１３Ｃ化学位移作了归属，并首次获得碱Ⅴ的晶体Ｘ衍射结构数据。Eight alkaloids were isolated frorn thebulb of Fritillaria Wabuensis S.Y. Tang et S.C. Yuehthe best“Chuan Bei”cultivated in Sichuan province,which is efficacious against cough.Five of them areknown,identified as imperialine (Ⅰ) , peimisine(Ⅱ),ebeinone(Ⅲ),isoverticine(Ⅳ),and imperialine-β-N-oxide(Ⅴ). All the signals for both 1H and 13C-NMR spectra of base(Ⅴ)were assigned by 2D NMRexperiments,and an X-ray structural data of alkaloid(Ⅴ)was obtained for tthe first time.

关 键 词：瓦布贝母 西贝素 贝母辛 X-衍射 分离 鉴定 

分 类 号：R284.2[医药卫生—中药学]