人胚胎提取液生物学活性研究及临床应用展望  

A Study of Biological Effects of Human Fetal Extract Both in vitro and vivo

作　　者：宋燕爽 杨毅[1] 李川[1] 刘洪隐[1] 

机构地区：[1]天津市肿瘤研究所放射生物室

出　　处：《中国肿瘤临床》1996年第2期92-96,共5页Chinese Journal of Clinical Oncology

摘　　要：本项研究证实从４～６．５个月的胎儿组织提取的有效成份（简称ＨＦＥ）具有多种生物学活性：可促进受照射损伤的小鼠造血功能的恢复，４项检测指标包括：①骨髓有核细胞数：实验组：６５．５±１５．８，对照组：１６．４±６．２。②外周血网织红细胞百分比Ｘ±ＳＤ，实验组：１６．６±０．６０，对照组０．２±０．０４。③ＣＦＵ－ＳＸ±ＳＤ，实验组：１９．６±９．６６，对照组：１．９±２．２８。④骨髓增生程度：实验组优于对照组。对５个肿瘤细胞株和正常人二倍体细胞株２ＢＳ均有不同程度的抑制生长作用，ＥＤ５０分别为Ｈｅｌａ：６２．５μｇ／ｍｌ，ＭＣＦ－７：７．８μｇ／ｍｌ，ＨＥＰ－２：４μｇ／ｍｌ，ＳＭＭＣ７７２１：６２．５μｇ／ｍｌ，Ｔｅａ：４．５μｇ／ｍｌ，而对ＬＡ－７９５和Ａ－５４９则有轻度刺激作用。可刺激人外周血单个核细胞增殖并与ＰＨＡ，ＩＬ－２有协同作用。对氨甲喋呤引起的大鼠肝、肺损伤有一定的保护治疗作用。本项研究将为其临床应用提供实验依据。This study confirmed that the human fetal extract(HFE) of 4-6.5months old embryo tissue had various biological activities:(1)promoted the recovery of damaged hemopoietic system in mice caused by X ray as evidenced by a)number of bone marrow nucleated cells,b)peripheral reticulocyte count,c)CFU-S,d) proliferation of bone marrow in femur;(2) inhibited growth of several tumor cells;(3)enhanced peripheral blood lymphocyte(PBL)proliferation synergistically with PHA or/and rIL-2,(4)promoted regeneration of the damaged tissue caused by methotrexate.

关 键 词：胚胎提取液 HFE 生物学活性 CFU-S MTT Effect 

分 类 号：R392－33[医药卫生—免疫学]