反相高效液相色谱法测定棒柄花中反式-4-(1-丙烯基)苯酚-β-D-吡喃葡萄糖苷  

Determination of Trans-4-(1-propenyl)-phenol-β-D-glucopyranoside in Cleidion brevipetiolatum Pax et Hoffm by Reversed-phase High Performance Liquid Chromatography

作　　者：刘布鸣[1] 卢文杰[1] 林霄[1] 陈家源[1] 牙启康[1] 

机构地区：[1]广西中医药研究所,广西南宁530022

出　　处：《分析测试学报》2006年第3期98-99,102,共3页Journal of Instrumental Analysis

基　　金：广西科技攻关项目(桂科攻0322024-5A);广西大型仪器协作共用专项资金资助项目(115-2003-51)

摘　　要：用反相高效液相色谱法建立棒柄花中反式-4-（1-阿烯基）苯酚-β-D-吡喃葡萄糖苷的含量测定方法，并测定其含量。色谱柱Reliasil-C18（250mm×4．6mm，5μm），流动相乙腈-水（体积比22：78），流速1．0mL／min．检测波长257nm。结果待测组分在0．0804—0．2814μg范围内与峰面积线性关系良好，相关系数为0．9995，平均加标回收率为98％，RSD为1．8％（n=6）。本法简便、快速、重复性好、结果准确可靠。A reversed-phase HPLC method was established to determine trans-4-（ l-propenyl）-phenol-β- D-glucopyranoside in Cleidion brevipetiolatum Pax et Hoffm. The chromatographic separation was accomplished using a Reliasil C18 column（250 mm ×4. 6 mm, 5 μm） with acetonitrile -water（22 ： 78 by volume） as mobile phase at a flow rate of 1.0 mL/min and a UV detector at λ257nm. A linear calibration curve was obtained for trans-4-（ 1-propenyl ）-phenol-β-D-glucopyranoside over the range of 0. 080 4 - 0. 281 4 μg with a correlation coefficient of 0. 999 5. The average recovery, was 98% and the relative standard deviation was 1.8% （ n = 6）. This method is rapid, reliable, simple and accurate.

关 键 词：棒柄花 反式-4-(1-丙烯基)苯酚-β-D-毗喃葡萄糖苷 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]