甲基维生素B_(12)对反复前脑缺血再灌注后的脑保护作用  

Cerebral protective effect of methylcobalamine in rats after frontal cerebral ischemia and reperfusion

作　　者：于挺敏[1] 满玉红[1] 吴杰[1] 

机构地区：[1]吉林大学第二医院神经内科,吉林长春130041

出　　处：《吉林大学学报（医学版）》2006年第3期390-392,F0002,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省计划委员会资助课题(2002年)

摘　　要：目的:探讨甲基维生素B12的脑保护作用机制。方法:双侧颈总动脉夹闭法制备反复前脑缺血再灌注模型。雄性Wistar大鼠45只,随机分为假手术组、缺血再灌注组及缺血再灌注甲基维生素B12治疗组(n=15),每组按缺血再灌注时间1、2、4周3个时间点再分为3组(n=5)。光镜HE染色、LFB髓鞘染色及电镜下观察海马、髓鞘的病理变化。结果:缺血再灌注组1周时光镜、电镜下可见海马神经元坏死,2和4周更加明显,LFB髓鞘染色见髓鞘脱失变性,4周较2周改变更明显,电镜下髓鞘鞘层模糊,部分溶解、折叠。甲基维生素B12治疗组海马神经元接近正常,髓鞘排列致密、有序,4周时改变最明显。结论:甲基维生素B12能明显减轻海马神经元和神经纤维髓鞘的缺血性损伤,具有脑保护作用。Objective To observe the pathological changes of hippocampus and myelin in rats with frontal eereberal ischemia and reperfusion after treated with methylcobalamine and to discuss the mechanism of the cerebral protective effect of methylcobalamine. Methods Cerebral ischemia and reperfusion models were made by clamping bilateral carotid arteries. Forty-five ＇male Wistar rats were randomly allocated to sham-operation group, isehemia reperfusion group and methylcobalamine group （n= 15）. 1, 2, 4 weeks after reperfusion the rats in each group were divided into three groups （n = 5）. The pathological changes of hippocampus and myelin were observed by using light microscope, HE staining, LFB staining and electron microscope. Results The pyramidal neurons in hippocampus in ischemia and reperfusion groups expressed putrescence and myelin necrosis or demyelination at /st week, and the histomorphological changes at 4th week were significant than that at 2nd week. The followings were observed in electron microscope： dimness, partly solubilization and collapsablity in myelin. The pyramidal neurons in hippocampus of therapy groups were similar to normal one and myelin expressed compaction and shipshape. The changes were more obvious at 4th week. Conclusion Methylcobalamine can obviously decrease the ischemia lesion of hippocampus and myelin and protect the brain function

关 键 词：维生素B12 海马 髓鞘 脑缺血 再灌注损伤 

分 类 号：R332[医药卫生—人体生理学] R743.31[医药卫生—基础医学]