镧对UV-B胁迫下大豆幼苗膜脂过氧化影响  

EFFECT OF LANTHANUM ON MEMBRANE LIPID PREOXIDATION OF SOYBEAN SEEDLINGS UNDES SUPPLEMENTAL UV-B IRRADIATION

作　　者：闫生荣 张岚 周青[2] 

机构地区：[1]江南大学环境生态研究室,无锡214036 [2]江南大学工业生物技术教育部重点实验室,无锡214036

出　　处：《大豆科学》2006年第2期145-148,共4页Soybean Science

基　　金：国家发改委稀土专项基金(GFZ040628);江苏省科技厅高新技术项目基金(GB2005040)资助

摘　　要：采用水培实验方法进行LaCl3对大豆(Clycine max)幼苗最适喷施浓度的筛选,并在此基础上初步研究了La对紫外辐射(UV-B:0.15W.cm-2,0.30W.cm-2)胁迫下大豆幼苗膜脂过氧化的影响.实验结果表明,20mg.L-1La能显著提高大豆叶片CAT、POD等抗氧化酶活性,降低细胞质膜透性和MDA含量,改善大豆幼苗抗氧化代谢功能.La+UV-B和UV-B组细胞质膜透性和MDA含量的动态走势相近,仅为前者的增幅低于后者,La+T1组的增幅低于La+T2,表明La能够缓解UV-B辐射造成的膜脂过氧化作用,且La对T1的防护效果优于T2。Effect of lanthanum （La^3＋ ） on membrane lipid peroxidation of soybean seedlings under elevated ultraviolet-B radiation （UV-B;280-330nm） at 15μW/cm^2 and 30μW/cm^2 level was studied with hydroponics under laboratory conditions. Firstly, such indexes of plasmolemma permeality, MDA content, CAT and POD activities were utilized to choose the best spraying concentration of rare earth to soybean seedlings. Our result showed that 20mg·L-1La significantly increased activities of CAT and POD, decreased plasmolemma permeality and MDA content and that improved antioxidant metabolism of soybean seedlings. The plasmolemma permeality and MDA content of soybean seedlings gradually increased during supplemental ultraviolet-B radiation and then decreased after ultraviolet-B stress. The dynamic trend of plasmolemma permeality and MDA content in soybean seedlings exposed to ultraviolet-B radiation with the pretreatment of lanthanum was similar to the above UV-B treatment. However, the range of variation of UV-B and La treatment was lower than only UV-B treatment; that of low dosage of UV-B and La treatment was also lower than high dosage of UV-B and La treatment. It could be concluded that La could mitigate membrane lipid peroxidation caused by UV-B stress and that its protective effects against low UV-B radiation was better than that of high UV-B radiation.

关 键 词：LA UV-B辐射 大豆幼苗 膜脂过氧化 

分 类 号：S565.1[农业科学—作物学]