血红素氧合酶-1高表达抗兔心肌缺血再灌注损伤研究  

Study of heme oxygenase-1 overexpression protection of rabbit heart from ischemia reperfusion injury

作　　者：黄维义[1] 张英[2] 严丽[1] 彭永权[1] 陈蓉[2] 

机构地区：[1]泸州医学院附属医院心内科,四川泸州646000 [2]泸州医学院病理生理教研室,四川泸州646000

出　　处：《中国现代医学杂志》2006年第10期1477-1479,1482,共4页China Journal of Modern Medicine

摘　　要：目的探讨血红素氧合酶-1(HO-1)高表达抗兔心肌IR损伤的效果及其作用机制。方法32只兔随机均分为单纯IR组与HO-1诱导组,分别预先给予生理盐水5mL及血晶素25mg/kg腹腔内注入,24h后,开胸结扎冠脉左前降支缺血40min,松开再灌注4h,测定心肌中HO-1表达及活性、中性粒细胞浸润、丙二醛(MDA)含量、心肌梗死范围及心肌细胞凋亡指数。结果与单纯IR组比较,HO-1预诱导组心肌中HO-1表达及活性均明显升高(P<0.01),中性粒细胞浸润及MDA含量明显降低(P<0.01),心肌梗死范围及心肌细胞凋亡指数均显著减少(P<0.01)。结论HO-1可通过抗炎、抗氧化等作用减轻急性心肌IR损伤。[Objective] To explore the effects and mechanism of heme oxygenase-1 （HO-1） overexpression protection of rabbit heart from ischemia reprfusion（IR） injury. [Methods ] 32 rabbits were randomly divided into simple IR group and HO-1 induced group, each rabbit was intra-peritoneal injections of 5 mL saline in simple IR group and 25mg/kg heroin in HO-1 induced group respectively. 24 hours later, rabbit heart underwent ischemia for 40 minutes by ligatin left anterior descending coronary artery and reperfusion for 4 hours. The myocardial HO-1 expression and HO-1 activity, polymorphonuclear neutrophils（PMN） infiltration, MDA content, infarct size and the apoptosis index（AI） in both groups were detected. [Results] The amount and activity of myocardial HO-1 of HO-1 induced group were significantly higher than that of simple IR group （P 〈0.01 respectively）. While the myocardial PMN infiltration, MDA content, infarct size and the AI of HO-1 induced group were much lower than that of simple IR group （P 〈0.01, respectively）. [Conclusion] HO-1 can reduce myocardial IR injury by means of anti-oxidative and anti-inflammatory properities.

关 键 词：血红素氧合酶-1 再灌注损伤 凋亡 中性粒细胞 兔 

分 类 号：R446.6[医药卫生—诊断学]