某部驻区螨类感染肾综合征出血热病毒的分子流行病学调查  

Survey on the molecular epidemiology of hantanviruse carried by mites in a base

作　　者：郝永建[1] 郭宝石[1] 邓致荣[1] 宋世佩[1] 郑金福[1] 白杨[1] 

机构地区：[1]第二炮兵防护防疫队,北京100071

出　　处：《医学动物防制》2006年第5期337-340,共4页Journal of Medical Pest Control

摘　　要：目的:了解某部驻区螨类携带肾综合征出血热病毒状况和病毒型别。方法:采集鼠类体表寄生的螨类。应用逆转录-聚合酶链反应(RT-PCR)扩增螨类模板的M片段基因序列,用BLAST软件进行序列分析,并用Clustal X(1.8)进行聚类分析。结果:RT-PCR检测结果显示,1组螨类标本中检测出了汉城型汉坦病毒,对一份阳性标本进行序列测定并分析其核苷酸序列,结果与中国黑龙江的AF28829株、日本的HANG12GP株、朝鲜的HVU38177株和HVU37729株同源性较高。结论:该部驻区鼠类体表寄生的螨类携带汉城型汉坦病毒。Objective： To investigate hantanviruse infection of mites in a base and to type hantanviruse using molecular technique. Methods. The captured mites were classified to fifteen groups. Reverse transcription-polymerase chain reaction（RT--PCR） technique was used to amplify the partial M fragments of hantanviruse. A representative positive sample was sequenced and analysed by Genbank BLUST and Clustal X（1.8） software. Results：The SEO--type of hantanviruse was detected from one group. Nucleotide sequences analysis showed 97% similarity with the nucleotide sequences of AF28829, HANG12GP, HVU38177 and HVU37729. Conclusions.The SEO--type of hantanviruse was carried by mites in the base.

关 键 词：螨类 肾综合征出血热病毒 分子流行病学 序列分析 

分 类 号：R373.32[医药卫生—病原生物学] R181.8[医药卫生—基础医学]