慢性肝病患者血清TIMP-1和TIMP-2的变化及其临床意义  

The change and significance of serum TIMP-1 and TIMP-2 levels in patients with chronic hepatitis B and liver cirrhosis.

作　　者：周建方[1] 何建方[2] 戴利成[2] 刘江[1] 王伟洪[1] 钱福初[2] 

机构地区：[1]湖州市中心医院感染内科,浙江湖州313000 [2]湖州市中心医院中心实验室,浙江湖州313000

出　　处：《检验医学》2006年第3期248-250,共3页Laboratory Medicine

摘　　要：目的探讨慢性乙型肝炎(CHB)和乙型肝炎后肝硬化(LC)患者血清中的基质金属蛋白酶-1组织抑制因子(TIMP-1)、基质金属蛋白酶-2组织抑制因子(TIMP-2)的变化及其临床意义。方法用酶联免疫吸附试验(ELISA)检测75例CHB患者和41例乙型肝炎后LC患者血清TIMP-1、TIMP-2含量,并与30名健康献血员比较。结果CHB轻度、中度、重度和LC A级、B级、C级患者血清TIMP-1均明显高于正常对照组(P均<0.01),与透明质酸(HA)、层黏连蛋白(LN)、Ⅳ型胶原(CⅣ)呈正相关(r=0.935、0.836、0.910,P均<0.01);CHB重度和LC A级、B级、C级患者血清TIMP-2均明显高于正常对照组(P均<0.01),与HA、LN、CⅣ呈正相关(r=0.765、0.623、0.716,P均<0.01)。结论血清TIMP-1、TIMP-2在慢性肝病患者肝纤维化过程中明显升高,并与肝纤维化指标呈正显著性相关,可作为判断肝纤维化程度的指标。Objective To investigate the change and significance of serum tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） and tissue inhibitor of metalloproteinase-2 （ TIMP-2 ） in patients with chronic hepatitis B （ CHB ） and liver cirrhosis （LC）. Methods Serum TIMP-1 and TIMP-2 were detected by enzyme-linked immunosorbent assay （ELISA） in 75 patients with CHB and 41 patients with LC, and 30 healthy persons served as normal control. Results The serum TIMP-1 concentrations in all patients with CHB or LC were significantly higher than those in normal control, and were positively related to the levels of hyaluronic acid （ HA ） , laminin （ LN ） and collagen type Ⅳ （ C Ⅳ ）. However the change of serum TIMP-2 concentration in patients with moderate degree CHB and LC was less significant. Condusions The serum levels of TIMP-1 and TIMP-2 are significantly increased in CHB and LC, and related to the levels of HA, LN and CⅣ measurement of them especially TIMP-2 can be used as diagnostic indices for liver fibrosis in chronic liver disease.

关 键 词：基质金属蛋白酶-1组织抑制因子 基质金属蛋白酶-2组织抑制因子 慢性乙型肝炎 肝硬化 

分 类 号：R446.62[医药卫生—诊断学]