巢式PCR扩增SRY基因序列鉴定牛胚胎性别的研究  

Study on Sex Determination of Bovine Pre-implantation Embryos by Nested PCR Amplifying the Sequence of SRY Gene

作　　者：白文林[1] 尹荣焕[1] 罗光彬[1] 孙明亮[1] 周晨阳[2] 纪英卓[1] 姜午旗[1] 张宝路[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110161 [2]辽宁省家畜家禽遗传资源保存利用中心,辽宁辽阳111000

出　　处：《安徽农业科学》2006年第8期1589-1590,共2页Journal of Anhui Agricultural Sciences

基　　金：辽宁省教育厅科学研究计划基金资助项目(2005358);沈阳农业大学青年教师科研基金资助项目(2004!009)。

摘　　要：源于牛X和Y染色体同源区的1对外引物P1、P2及其各自特异区的2对内引物P3、P4和P5、P6进行巢式PCR反应,对中国荷斯坦公、母牛静脉血样及牛胚胎样进行特异性条带鉴定。结果表明,中国荷斯坦公牛出现178bp和262bp2条带,而母牛仅出现262bp1条带;静脉血样与实际性别相符率为100%;巢式PCR胚胎性别鉴定技术方法准确、简便,具有很高的灵敏性及稳定性。The specific band of sex was identified through the bull, cow blood sample and pre-implantation embryos of China Holsteins by outer primers P1, P2 from complementary region of X chromosome and Y chromosome, inner primers P3, P4 and PS, P6 from specific region of X chromosome and Y chromosome. The result showed that bull demonstrated two band of 178 bp and 262 bp, and cow only demonstrated a band of 262bp, The result of blood identification was completely in accordance with the actual sex type. The sex determination of 11 embryos with the nested PCR method from China Holsteins showed that this method was effective and sensitive.

关 键 词：牛胚胎 巢式PCR 性别鉴定 

分 类 号：S823.3[农业科学—畜牧学]