采用DNA修饰的金电极表面电化学方法对阿霉素与DNA相互作用的研究(英文)  

DNA modified gold surface electrochemical method for determining the interaction of adriamycin with DNA

作　　者：周宪梁 张慧敏 傅春燕 甄一松 邹玉宝 刘玉清 惠汝太 

机构地区：[1]Cardiovascular Institute and Fu Wai Cardiovascular Hospital,Peking Union Medical College,Chinese Academy of MedicalSciences,Beijing100037,Both authors contribute to the work equally [2]Institute of Chemistry,Chinese Academy of Sciences,Beijing100080,China,Both authors contribute to the work equally [3]Cardiovascular Institute and Fu Wai Cardiovascular Hospital,Peking Union Medical College,Chinese Academy of MedicalSciences,Beijing100037

出　　处：《中国分子心脏病学杂志》2004年第4期199-203,共5页Molecular Cardiology of China

基　　金：国家重点基础性研究项目 (G2 0 0 0 0 5690 2 )~~

摘　　要：众所周知 ,阿霉素是一种抗肿瘤药物 ,并能引起严重的心脏副作用。它的疗效和副作用被认为是由于它与DNA之间存在有相互作用。人们已采用不同的方法对DNA和阿霉素的相互作用进行了研究 ,但是由于实验方法本身的限制和实验条件的不同 ,很难得到统一的结果。为了研究阿霉素和DNA之间相互作用的机制 ,我们采用DNA修饰的金电极表面电化学方法对阿霉素与DNA相互作用进行了研究 ,结果发现 ,在酸性溶液中 ,阿霉素带正电荷 ,dsDNA带负电荷 ,两者之间的相互作用主要是静电结合。随着pH值的增加 ,在中性溶液中 ,阿霉素与dsDNA的相互作用是嵌入结合。另外 ,在酸性溶液中 ,吸附在电极表面的ssDNA与阿霉素之间存在有静电作用。这些结果为深入理解阿霉素与DNA的相互作用提供了帮助。It has long been known that adriamycin is an antiturnor antibiotics with severe cardiac side effects. Both its therapeutic and side effects is believed to result from its interaction with DNA. Various methods have been used to study the interaction, but no consistent results could be generated due to problematic methods and experimental conditions. To investigate the physical interaction of adriamycin with DNA, we used a DNA modified gold surface electrochemical electrode method and found that in acidic solution, the electrostatic interaction plays the dominant role between positively charged adriamycin and negatively charged dsDNA. With pH increasing, in neutral solution, the interaction of dsDNA with adriamycin is intercalation. Additionally, it was found that in acidic solution, the ssDNA adsorbed on gold surface can also interact with adriamycin by electrostatic action. These results will be useful for further understanding the interaction of DNA with adriamycin.

关 键 词：DNA 阿霉素 循环伏安 DNA修饰电极 

分 类 号：R346[医药卫生—基础医学]