同型半胱氨酸对大鼠主动脉平滑肌细胞增殖及增殖细胞核抗原基因表达的影响  

Effects of Homocysteine on the proliferation of Rat Aortic Smooth Muscle Cell and the Expression of Proliferating Cell Nuclear Antigen(PCNA)/Cyclin

作　　者：周宪梁[1] 惠汝太 刘力生[1] 宋雷[1] 胡爱华[1] 刘玉清[1] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院高血压诊治中心,北京市100037

出　　处：《中国分子心脏病学杂志》2004年第4期235-237,共3页Molecular Cardiology of China

基　　金：国家重点基础性研究项目 (G2 0 0 0 0 5690 2 )

摘　　要：目的　研究同型半胱氨酸 (Hcy)对大鼠主动脉平滑肌细胞增殖及增殖细胞核抗原基因(cyclinD1)mRNA表达的影响。方法　采用同位素技术测定3H TdR参入法和核酸分子杂交技术检测大鼠主动脉平滑肌细胞增殖及增殖细胞核抗原基因 (cyclinD1)mRNA表达的水平。结果　Hcy能促进VSMC增殖 ,并且具有剂量效应关系 ,同时可增加cyclinD1mRNA表达水平 3 2倍。结论　Hcy促进VSMC增殖可能是通过增加cyclinD1mRNA表达水平的途径实现的 ,使静止期细胞进入细胞分裂周期 ,从而促进VSMC迅速增殖。Objective To study the effect of homocysteine （Hcy）on the proliferation of vascular smooth muscle ceils （VSMC）and the expression of cyclin D1 mRNA. Methods 3^H-TdR incorporation and Northern blot were assayed to detect the amount of 3^H-TdR and the levels of cyclin D1mRNA expression. Results Hcy induced the proliferation of VSMC and there was a dose-dependent relationship in it. Hcy increased the expression of cyclin D1 mRNA in rat aortic smooth muscle cells by 3.2-fold. Conclusion Hcy induced the proliferation of VSMC probably by the increased the expression of cyclin D1mRNA, indicating the Hcy induced the mRNA of cyclin is important for the reentry of quiescent VSMC into the cell cycle.

关 键 词：同型半胱氨酸 动脉粥样硬化 危险因素 细胞周期 

分 类 号：R543[医药卫生—心血管疾病]