小麦高分子量谷蛋白亚基1Ax2`的AS-PCR鉴定  

Identification of 1Ax 2 Subunit of Wheat HMW Glutenin Using AS-PCR

作　　者：孙宪印[1] 吴科 钱兆国 卫宪云[1] 米勇 李斯深[1] 

机构地区：[1]山东农业大学农学院,山东泰安271018 [2]山东省泰安市农科院,山东泰安271000

出　　处：《麦类作物学报》2006年第3期75-77,共3页Journal of Triticeae Crops

基　　金：农业结构调整重大技术研究专项(04-02-05B);科技部农业科技成果转化资金项目(2004-309)。

摘　　要：为了从DNA水平上快速鉴定Glu-A1位点编码的1Ax2*优质亚基,建立了稳定的检测1Ax2*优质亚基基因的PCR扩增体系。通过该体系,1Ax2*优质亚基基因扩增出2652bp特异片段,而其他亚基基因则不能扩增出该片段。验证材料的PCR鉴定结果与SDS-PAGE电泳结果一致,表明利用该体系鉴定1Ax2*亚基是可行的。利用此体系鉴定了44份外引小麦品种(系)的谷蛋白Glu-A1位点,有24个品种(系)含有1Ax2*亚基,1Ax2*亚基出现频率为54.5%。In order to identify the 1Ax2^＊ subunit of high molecular weigh （HMW） glutenin coded by Glu-A1 locus at the DNA level, we constructed a stable ASPCR amplified system of detecting 1Ax2^＊ subunit gene, which 1Ax2^＊ subunit gene could be amplified as a 2 652bp fragment, while other sub- unit genes on GluA1 locus could not be amplified. The result of AS-PCR identification was accordant with SDS-PAGE identification for testing varieties, showed that the AS-PCR system can be used in detecting of 1Ax2^＊ subunit. We used this AS-PCR method to detect 44 foreign wheat varieties （lines）. The results showed that 24 varieties（lines）（54. 5%） had 1Ax2^＊ subunit and 20 varieties （lines） had not.

关 键 词：小麦 1Ax2^＊ 等位基因特异性PCR 

分 类 号：S512.1[农业科学—作物学] S331