维甲酸诱导人成骨肉瘤MG-63细胞分化过程中核基质构型及其蛋白质组成变化观察  

Observation of the Effects of Retinoic Acid on the Configurational Changes of the NM-IF System and the Alteration of Nuclear Matrix Proteins in Human Osteosarcoma Cell Line MG-63

作　　者：赵妍[1] 唐剑[1] 赵春红[1] 石松林[1] 李祺福[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学研究室细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第B05期6-10,共5页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(30470877);福建省自然科学基金(C0310003)资助

摘　　要：应用选择性抽提、整装透射电镜观察和双向聚丙烯酰胺凝胶电泳技术,分析维甲酸诱导处理前后人成骨肉瘤MG-63细胞核基质-中间纤维系统的构型变化,以及核基质蛋白表达变化.实验结果显示MG-63细胞核基质和中间纤维数量较少、分布不均匀,核纤层为薄厚不一结构,与两类纤维联系不密切.经1μmol/L维甲酸处理后,细胞核基质纤维和中间纤维数量增多、结构层次丰富、单丝成分多,分布均匀并相互交织成规则网络,两类纤维通过薄层均一的核纤层发生密切联系,形成贯穿整个细胞核质区域的完整体系.此外,核基质蛋白表达也发生显著变化.表明经维甲酸诱导处理后MG-63的核基质-中间纤维系统产生了与正常细胞相似的恢复性改变,并且伴有核基质蛋白表达的差异.这些变化是癌细胞恶性表型逆转的重要特征和功能表现,对于揭示核基质构型及其蛋白组成与细胞癌变和逆转的关系和阐明细胞增殖分化的基因表达调控原理,均具有重要意义.The configurational changes of nuclear matrix-intermediate filament （NM-IF） system and the alteration of the nuclear matrix proteins （NMPs） during differentiation of Osteosarcoma cell line MG-63 after being induced by Refinoic Acid （RA） have been investigated by selective extraction, whole-mount optic and transmission electron microscopy and two-dimensional gel electrophoresis. It was revealed that the NM and IF filaments in MG-63 cells was relatively few and scattered, not well distributed and arranged irregularly. The nuclear lamina was not uniformly thick, and connected to NM and IF filaments relaxedly. After being induced by 1 μmol/L RA, the two kinds of filaments were plentiful and well-distributed, differed in slender, thick form and interweaved into a regular network. The NM and IF filaments connected closely to the thin and compact fiber-like lamina, and formed a regular network through the whole cell region. And the NMPs were altered at the same time. It was demonstrated that the NM-IF system in MG-63 cell had been undergone a restorational change similar to that of normal cells after RA treatment. And inducing differentiation of MG-63 cells is companied with the expression differentia of NMPs. This alternation is an important morphological and functional expression to the malignant phenotypic reversion of cancer cells. And it has an important significance to reveal the relationship between nuclear matrix proteins and cell carcinogenesis and reversion, and to elucidate the regulational principle of gene expression in cell proliferation and differentiation.

关 键 词：核基质-中间纤维 核基质蛋白 人成骨肉瘤细胞 维甲酸 

分 类 号：Q247[生物学—细胞生物学]