OsICK1基因植物反义表达载体的构建及遗传转化  

Construction of OsICK1 Plant Antisense Expression Vector

作　　者：曾雅明[1] 何艺宾[1] 张红心[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第B05期66-69,共4页Journal of Xiamen University：Natural Science

摘　　要：O sICK1是水稻细胞周期蛋白依赖性激酶抑制剂基因.我们构建了水稻细胞周期蛋白依赖性激酶抑制剂基因(O s-ICK1)的植物反义表达载体,在农杆菌的介导下尝试使antisenseO sICK1整合到水稻基因组中并初步得到验证.为进一步研究O sICK1对水稻细胞周期调控的作用奠定了基础.Cyclin-dependent protein kinases （CDKs） have a central role in cell cycle regulation. It can be inhibited by the binding of small protein CDK inhibitors （ICKs）. OsICK1 is a putative cyclin-dependent protein kinase inhibitor gene found in rice. The aim of this research was to study the fuedon of OsICK1 by expressing antisence OsICK1 in rice. First, we constructed a plant OsICK1 antisense expression vector under the control of CaMV35S promotor and NOS terminator. Then, the antisense gene was integrated into the genome of rice callus via Agrobacterium tumefaciens EHA105 mediation. It provided a reliable base for the function study on OsICK1.

关 键 词：OsICK1 表达载体 反义 

分 类 号：S641.2[农业科学—蔬菜学] S566.2[农业科学—园艺学]