人角质细胞生长因子基因转化水稻的初探  

Study on Introducing KGF Gene into Rice Mediated by Agrobacterium

作　　者：苏勇波[1] 张红心[1] 沈明山[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第B05期70-72,共3页Journal of Xiamen University：Natural Science

摘　　要：角质细胞生长因子(Keratinocyte growth factor,KGF)在组织的损伤修复中起着重要的作用.在植物里面表达高活性的KGF具有成本低、易开发的优点.本实验将人角质细胞生长因子基因经农杆菌介导转入水稻中,获得110株再生植株.对再生植株进行GUS检测发现有44.7%植株呈阳性,PCR检测的结果也显示有52.6%的植株为阳性植株.Keratinocyte growth factor （KGF） plays an important role in the wound repair. It is economical to express KGF in plant. In this study, we introduced human KGF gene into rice mediated by Agrobacterium. 110 independent seedlings regenerated from transformants have been obainted. The positive ratio of the seedling is 44.7% by GUS detection and 52.6% by PCR detection.

关 键 词：角质细胞生长因子 遗传转化 基因表达 

分 类 号：Q78[生物学—分子生物学]