水稻OsICK1基因克隆及其序列分析  

Cloning and Sequence Analysis of Cyclin-dependent Kinase Inhibitor Gene in Oryza sativa

作　　者：何艺宾[1] 曾雅明[1] 王蕾[1] 沈明山[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第B05期78-81,共4页Journal of Xiamen University：Natural Science

摘　　要：细胞周期蛋白依赖性激酶抑制剂(ICK)是一种能够影响细胞生长和发育的重要调控蛋白.以水稻(9311)为材料,利用RT-PCR技术,扩增获得了1个新的可能为水稻细胞周期蛋白依赖性激酶抑制剂的基因,命名为O sICK1.核苷酸序列分析表明,该基因的开放阅读框为585 bp,编码194个氨基酸.与Genbank中预测的水稻ICK基因序列(NM_196964)比对,两者同源性为79.84%;氨基酸序列分析表明,该基因氨基酸序列3′端附近存在植物ICK所固有的保守序列,与玉米ICK1保守序列的同源性高达95.5%.Cyclin-dependent kinase inhibitor （ICK） is a key control protein in Cell cycle that can affect the growth and development of call. By RT-PCR, the likely gene of cyclin-dependent kinase inhibitor in Oryza sativa （9311） was cloned and named OsICK1. Results of Nucleotide sequence analysis demonstrated that the ORF of the gene was 585 bp, encoded a protein consisting of 194 amino acids. Compared to the putative ICK sequence of Oryza sativa （japonica cultivar-group） in Genbank, the rate of homology was 79.84%. Results of amino acid sequence analysis indicated that the 3＇ end of protein had a conserved domain of Plant ICK. Compared to the conserved domain of Zeama; KRP; 1, the rate of homology was highly 95.5%.

关 键 词：水稻 细胞周期蛋白依赖性激酶抑制剂 基因克隆 

分 类 号：Q946.2[生物学—植物学]