双向电泳联用质谱技术研究水稻明恢63对细菌性条斑病侵染的应答  

Analysis of the Defense Responsiveness in Hybrid Rice Minghui63 Against Bacterial Leaf Streak(Xanthomonas oryzae pv.Oryzicola) Using Two-dimensional Electrophoresis and Mass Spectrometry

作　　者：黄青云[1] 林涛[1] 陈芳育[2] 张红心[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]生物农药与化学生物学教育部重点实验室,福建农林大学作物科学学院,福建福州350002

出　　处：《厦门大学学报（自然科学版）》2006年第B05期86-90,共5页Journal of Xiamen University：Natural Science

基　　金：生物农药与化学生物学教育部重点实验室(福建农林大学)开放课题基金(KF0411)资助

摘　　要：水稻细菌性条斑病是由细条病菌Xanthom onas oryzaepv.oryzicola(Xooc)引起的一种细菌性病害,是我国南方稻区的主要病害.为了探明水稻对细条病的应答机制,我们应用双向电泳联用质谱技术,研究了水稻品种明恢63在水稻细条病病菌侵染后不同时期的全蛋白变化,并对差异蛋白进行了鉴定以及分析归类.共鉴定出17种表达上调的蛋白和10种表达下调的蛋白,这些蛋白参与了水稻对细条病侵染的信号识别及防卫应答,其中包括信号转导类蛋白、防卫相关蛋白、代谢相关蛋白和参与蛋白质合成的蛋白等.Rice bacterial leaf streak （BLS） caused by pathogen Xanthomonas oryzae pv. oryzicola （Xooc） is one of the major rice diseases in South China. For proving up the defense mechanism of rice against Bacterial Leaf Streak, the differentially express proteins of Minghui 63 leaf after BLS infection in different inoculation time were analysised by using the two-dimensional electrophoresis and mass spectrometry. 17 up-regulated proteins and 10 down-regulated proteins were identified. These proteins involvd in signal recognition and defense response, including signal transduce proteins, defense associated proteins, metabolism associated proteins and protein synthesis associated proteins.

关 键 词：水稻 细菌性条斑病 双向电泳 MALDI-TOF/MS 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]