H5N1型禽流感病毒HA基因在烟草中的表达  

Expression of H5N1HA Gene of an Avian Influenza Virus in Tobacco

作　　者：郭小玲[1] 崔红[2] 王宇[3] 张国广[3] 陈亮[3] 

机构地区：[1]厦门大学海洋与环境学院,福建厦门361005 [2]河南农业大学国家烟草栽培生理生化基地,河南郑州450000 [3]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第B05期126-128,共3页Journal of Xiamen University：Natural Science

基　　金：福建省科技厅重点项目(2004N005)资助

摘　　要：禽流感病毒H5N1是可以直接感染人类的甲型流感病毒,发展植物源口服疫苗是疫苗研究的方向之一.本研究通过农杆菌介导的方法将禽流感病毒H5N1的HA基因转化烟草.共获得38株潮霉素抗性植株,经PCR和Southern-b lot-ting检测,目的基因已整合到转基因植株的基因组中.W estern-dotting检测结果表明,目的基因在转基因烟草中得到表达,具有免疫原性,获得了能够表达HA基因的植物口服疫苗候选植株.H5N1, a novel avian influenza virus, can infect human directly. In this study, the DNA fragment of H5N1 avian influenza HA gene, antigen determinant, was transferred into tobacco （Nicotiana tabacum cv.） via leaf-discs of Agrobacterium-mediated. Total 38 transgenic plants were regenerated. It was demonstrated that the fragment of H5N1 HA gene had been integrated into the genome of tobacco by PCR and Southern-blotting. The proteins expressed in transgenic plants had immunogenicity by Western-dotting analysis.

关 键 词：禽流感病毒 H5N1 烟草 转基因 

分 类 号：Q943.2[生物学—植物学]