湖北省犊牛致病性大肠杆菌病的检测与分离鉴定  

Examination,Separation and Identification of the Calf Pathogenic E.coli in Hubei Province

作　　者：胡智斌 宋念华 李德学 梁望旺[2] 张厚芝 谢军 田峰 蔡传鹏 周煜 

机构地区：[1]湖北省兽疫防治站,武汉430064 [2]湖北省农业科学院畜牧兽医研究所,武汉430064

出　　处：《湖北农业科学》2006年第3期362-364,共3页Hubei Agricultural Sciences

摘　　要：2005年6月,湖北某地耕牛暴发疫病,26头发病牛死亡14头,死亡牛以犊牛为主。经取病料分离、培养、纯化,接种小白鼠,挑纯培养菌落和取试验动物脏器检验,革兰氏染色镜检为革兰氏阴性短杆菌。用BioMerieuxATB全自动细菌鉴定仪,使用ID32E肠杆菌鉴定条I、D32GN革兰氏阴性菌鉴定条,鉴定为大肠埃希氏杆菌;大肠杆菌O抗原血清因子试验鉴定为O9型、O101型大肠杆菌。取ATBVET药敏条进行药敏试验,结果36种抗生素11种敏感。根据流行病学、临床症状、实验室检测等综合诊断,此次疫情确定为牛败血型大肠杆菌病。In June,2005, an epidemic disease occurred in Hubei province, and 26 cattle infected and 14 cattle died, Bacterioscopy demonstrated that the bacterium was G^- short bacillus, With the BioMerieux ATB expression, the bacterium was identified Escherichia coli. It was identified serotype 09 and O101 using the E.coli O-Agserumfactor experimentation. The medicine sensitive experiment showed that it was sensitive to the 11 kind of 36 antibiotics. According to epidemiology, clinical symptom and laboratory examination, this epidemic was diagnosed cattle-septicaemic-E.coli disease.

关 键 词：犊牛 大肠埃希氏杆菌 分离 鉴定 湖北 

分 类 号：S858.23[农业科学—临床兽医学] S852.612[农业科学—兽医学]